Anti-DDX27 抗体 [EPR13146(B)] (ab177938)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13146(B)] to DDX27
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DDX27 antibody [EPR13146(B)]
DDX27 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13146(B)] to DDX27 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Mouse, Human
非交差種: Rat -
免疫原
Synthetic peptide within Human DDX27 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96GQ7 -
ポジティブ・コントロール
- HepG2, HeLa, Jurkat and NIH 3T3 cell lysates; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13146(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177938の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 90 kDa.
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ICC/IF |
1/50 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 90 kDa. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Probable ATP-dependent RNA helicase. -
配列類似性
Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55661 Human
- Entrez Gene: 228889 Mouse
- SwissProt: Q96GQ7 Human
- SwissProt: Q921N6 Mouse
- Unigene: 404263 Human
- Unigene: 65234 Human
- Unigene: 295031 Mouse
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別名
- C86129 antibody
- cb843 antibody
- DDX27 antibody
see all
画像
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Immunofluorescent analysis of HeLa cells labeling DDX27 with ab177938 at 1/50 dilution.
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All lanes : Anti-DDX27 antibody [EPR13146(B)] (ab177938) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 90 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab177938 は 1 報の論文で使用されています。
- Jin Y et al. DEAD-Box Helicase 27 Triggers Epithelial to Mesenchymal Transition by Regulating Alternative Splicing of Lipoma-Preferred Partner in Gastric Cancer Metastasis. Front Genet 13:836199 (2022). PubMed: 35601484