Anti-DDX21 抗体 [EPR14496] (ab181870)
![Western blot - Anti-DDX21 antibody [EPR14496] (ab181870)](https://www.abcam.co.jp/ps/products/181/ab181870/Images/ab181870-212891-anti-ddx21-antibody-epr14496-western-blot.jpg "Western blot - Anti-DDX21 antibody [EPR14496] (ab181870)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14496] to DDX21
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-DDX21 antibody [EPR14496]
DDX21 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14496] to DDX21 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- HeLa, HT-1080 and HEK293 cell lysate
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14496 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181870の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa). |
ターゲット情報
-
機能
Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Functions as cofactor for JUN-activated transcription. Involved in rRNA processing. -
配列類似性
Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
ドメイン
The two enzymatic activities reside in two separate domains, the helicase in the N-terminus and the foldase in the C-terminus.
The 3 X 5 AA repeats seem to be critical for the RNA folding activity. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
-
参照データベース
- Entrez Gene: 9188 Human
- Omim: 606357 Human
- SwissProt: Q9NR30 Human
- Unigene: 223141 Human
-
別名
- Ddx21 antibody
- DDX21_HUMAN antibody
- DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody
see all
画像
-
Western blot - Anti-DDX21 antibody [EPR14496] (ab181870)All lanes : Anti-DDX21 antibody [EPR14496] (ab181870) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 87 kDa -
![Western blot - Anti-DDX21 antibody [EPR14496] (ab181870)](https://www.abcam.co.jp/ps/products/181/ab181870/Images/ab181870-212892-anti-ddx21-antibody-epr14496-western-blot.jpg)
Western blot - Anti-DDX21 antibody [EPR14496] (ab181870)Anti-DDX21 antibody [EPR14496] (ab181870) at 1/10000 dilution + HT-1080 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 87 kDa -
![Anti-DDX21 antibody [EPR14496] (ab181870)](https://www.abcam.co.jp/ps/products/181/ab181870/Images/ab181870-3-benefits-of-recombinant-antibodies.png)
Anti-DDX21 antibody [EPR14496] (ab181870)
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab181870 は論文での使用が確認できていません。