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AB70305

Anti-DDX19B 抗体

Anti-DDX19B antibody

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(2 Publications)

Rabbit Polyclonal DDX19B antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DDX19B.

別名を表示する

DBP5, DDX19, TDBP, DDX19B, ATP-dependent RNA helicase DDX19B, DEAD box RNA helicase DEAD5, DEAD box protein 19B

1 Images
Western blot - Anti-DDX19B antibody (AB70305)
  • WB

Unknown

Western blot - Anti-DDX19B antibody (AB70305)

All lanes:

Western blot - Anti-DDX19B antibody (ab70305) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 54 kDa

Observed band size: 53 kDa

false

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human DDX19B. The exact immunogen used to generate this antibody is proprietary information.

Q9UMR2

特異性

The immunogen sequence is highly conserved with DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

DDX19B also known as DEAD (Asp-Glu-Ala-Asp) box helicase 19B is an RNA helicase enzyme that assists in RNA metabolism by unwinding RNA molecules. It is part of the DEAD-box protein family characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). This protein has a molecular weight of approximately 48 kDa. DDX19B is expressed in various human tissues most prominently in the liver and brain. This wide expression pattern suggests its involvement in essential cellular processes.
Biological function summary

DDX19B plays a vital role in RNA processing and transport. It is involved in the export of mRNA from the nucleus to the cytoplasm a critical step in gene expression. DDX19B interacts with large protein complexes such as the nuclear pore complex which facilitates the movement of molecules across the nuclear envelope. By engaging in these interactions DDX19B ensures the proper communication between the nucleus and cytoplasm maintaining cellular homeostasis.

Pathways

DDX19B is integral to the mRNA export pathway working closely with proteins such as NUP214 a nucleoporin essential for the export process. Additionally DDX19B participates in the nonsense-mediated mRNA decay (NMD) pathway which controls mRNA quality by degrading erroneous mRNA molecules. In this pathway DDX19B interacts with proteins UPF1 a critical regulator of NMD reflecting its importance in maintaining proper gene expression and preventing accumulation of faulty proteins.

DDX19B has been linked to certain cancers such as liver cancer and neurodegenerative diseases like amyotrophic lateral sclerosis (ALS). In liver cancer DDX19B has shown aberrant expression patterns pointing to its potential role in tumorigenesis. In relation to ALS its interaction with FUS an RNA-binding protein associated with the disorder highlights its involvement in the disease's molecular mechanisms. These connections emphasize the need to further explore DDX19B as a therapeutic target in managing such conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

ATP-dependent RNA helicase involved in mRNA export from the nucleus (PubMed : 10428971). Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (PubMed : 10428971).
See full target information DDX19B
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 14: PubMed41036778

2025

Genome organization by SATB1 binding to base-unpairing regions (BURs) provides a scaffold for SATB1-regulated gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinori Kohwi,Xianrong Wong,Mari Grange,Thomas Sexton,Hunter W Richards,Yohko Kitagawa,Shimon Sakaguchi,Ya-Chen Liang,Cheng-Ming Chuong,Vladimir A Botchkarev,Ichiro Taniuchi,Karen L Reddy,Terumi Kohwi-Shigematsu

Nucleic acids research 49:8261-8276 PubMed34232997

2021

Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF-DDX19B interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonkyoung Park,Joori Park,Hyun Jung Hwang,Leehyeon Kim,Kwon Jeong,Hyun Kyu Song,Simone C Rufener,Oliver Mühlemann,Yoon Ki Kim
View all publications
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