Anti-DDX1 抗体 (ab70252)
Key features and details
- Rabbit polyclonal to DDX1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDX1 antibody
DDX1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDX1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human DDX1 (C terminal). Synthetic peptide corresponding to a region between residue 650 and the C-terminus (residue 697) of human DDX1. (AAH12739.1)
Database link: Q92499 -
ポジティブ･コントロール
- Whole cell lysate from Hela cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP | (1) |
Use at 3-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 3-5 µg/mg of lysate. |
ターゲット情報
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機能
Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs. -
組織特異性
Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord. -
配列類似性
Belongs to the DEAD box helicase family. DDX1 subfamily.
Contains 1 B30.2/SPRY domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
ドメイン
The helicase domain is involved in the stimulation of RELA transcriptional activity. -
翻訳後修飾
Phosphorylated. Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR). -
細胞内局在
Nucleus. Cytoplasm. Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev. - Information by UniProt
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参照データベース
- Entrez Gene: 395249 Chicken
- Entrez Gene: 1653 Human
- Entrez Gene: 104721 Mouse
- Entrez Gene: 84474 Rat
- Omim: 601257 Human
- SwissProt: Q92499 Human
- SwissProt: Q91VR5 Mouse
- SwissProt: Q641Y8 Rat
see all -
別名
- ATP dependent helicase DDX1 antibody
- ATP-dependent RNA helicase DDX1 antibody
- DBP RB antibody
see all
画像
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Western blot - Anti-DDX1 antibody (ab70252)All lanes : Anti-DDX1 antibody (ab70252) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 83 kDa
Exposure time: 1 second -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (ab70252)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian seminoma tissue labelling DDX1 with ab70252 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-DDX1 antibody (ab70252) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (ab70252)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal seminoma tissue labelling DDX1 with ab70252 at 1/200 (1µg/ml). Detection: DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab70252 は 2 報の論文で使用されています。
- Puri D et al. Comparative nuclear matrix proteome analysis of skeletal muscle cells in different cellular states. Cell Biol Int 45:580-598 (2021). PubMed: 33200434
- Wang IX et al. Human proteins that interact with RNA/DNA hybrids. Genome Res 28:1405-1414 (2018). PubMed: 30108179