Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 (ab1170)
Key features and details
- Chicken polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgY
製品の概要
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製品名
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to DDDDK tag (Binds to FLAG® tag sequence) -
由来種
Chicken -
アプリケーション
適用あり: ELISA, WB, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin. Peptide xxxDDDDK, xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
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特記事項
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide coupled to a different carrier. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1170の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
1/5000 - 1/20000.
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| WB | (2) |
1/2000 - 1/10000.
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| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
|
ELISA
1/5000 - 1/20000. |
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WB
1/2000 - 1/10000. |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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関連性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
別名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170)
Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed permeabilized with 0.1% Triton X-100 CHO cells (A) transfected with GFP-3xDYKDDDDK constructs (CHO-GFP-3xFLAG) or (B) non-transfected parental cells, labelling DYKDDDDK tag with ab1170 at 5 µg/mL, followed by Goat Anti-Chicken IgY (H&L), Alexa594 conjugated antibody at 2.5 µg/mL (Red). Parental CHO cells were used as a negative control. Nucleus was counterstained with DAPI (Blue).
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Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170)This image is courtesy of an anonymous AbreviewAnti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170) at 1/5000 dilution + Escherichia coli Recombinant protein (BL21)
Secondary
Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab1170 は 9 報の論文で使用されています。
- McGlacken-Byrne SM et al. Pathogenic variants in the human m6A reader YTHDC2 are associated with primary ovarian insufficiency. JCI Insight 7:N/A (2022). PubMed: 35138268
- Luttik K et al. Identifying Disease Signatures in the Spinocerebellar Ataxia Type 1 Mouse Cortex. Cells 11:N/A (2022). PubMed: 36078042
- Schmerl B et al. The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. PLoS Biol 20:e3001503 (2022). PubMed: 35312684
- Cao Y et al. SIRT3 promotion reduces resistance to cisplatin in lung cancer by modulating the FOXO3/CDT1 axis. Cancer Med 10:1394-1404 (2021). PubMed: 33655712
- Matsui H et al. Cytosolic dsDNA of mitochondrial origin induces cytotoxicity and neurodegeneration in cellular and zebrafish models of Parkinson's disease. Nat Commun 12:3101 (2021). PubMed: 34035300
- Guo Q et al. ZEB2, interacting with MDM2, contributes to the dysfuntion of brain microvascular endothelial cells and brain injury after intracerebral hemorrhage. Cell Cycle 20:1692-1707 (2021). PubMed: 34334113
- Menon A et al. Electrophysiologic and molecular mechanisms of a frameshift NPPA mutation linked with familial atrial fibrillation. J Mol Cell Cardiol 132:24-35 (2019). PubMed: 31077706
- Lim J et al. Nuclear Proximity of Mtr4 to RNA Exosome Restricts DNA Mutational Asymmetry. Cell 169:523-537.e15 (2017). PubMed: 28431250
- Hutson MR et al. Temperature-activated ion channels in neural crest cells confer maternal fever-associated birth defects. Sci Signal 10:N/A (2017). PubMed: 29018170