Anti-DDB1 抗体 (ab9194)
Key features and details
- Goat polyclonal to DDB1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DDB1 antibody
DDB1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DDB1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog
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免疫原
Synthetic peptide corresponding to Human DDB1 aa 1128-1140 (C terminal).
Sequence:C-DLIKVVEELTRIH
(Peptide available asab23152) -
ポジティブ･コントロール
- WB: HeLa lysate, HepG2 lysate, Jurkat lysate, NIH3T3 lysate and NSO lysate. IHC-P: Human cortex tissue.
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特記事項
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
Recognizes the large subunit of DNA damage-binding protein - functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). DDB-1 (damage-specific DNA binding protein 1) is also known as: DDBA; XAP1; XPCE; XPE-BF;UV-DDB1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9194の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 0.01 - 2 µg/ml. Predicted molecular weight: 127 kDa.
1 hour primary incubation is recommended for this product. |
| IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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WB
Use a concentration of 0.01 - 2 µg/ml. Predicted molecular weight: 127 kDa. 1 hour primary incubation is recommended for this product. |
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the DDB1 family. -
翻訳後修飾
Ubiquitinated by CUL4A. Subsequently degraded by ubiquitin-dependent proteolysis. -
細胞内局在
Cytoplasm. Nucleus. Primarily cytoplasmic. Translocates to the nucleus following UV irradiation and subsequently accumulates at sites of DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 511951 Cow
- Entrez Gene: 100290337 Human
- Entrez Gene: 1642 Human
- Entrez Gene: 13194 Mouse
- Entrez Gene: 64470 Rat
- Omim: 600045 Human
- SwissProt: A1A4K3 Cow
- SwissProt: Q16531 Human
see all -
別名
- Damage specific DNA binding protein 1 antibody
- Damage-specific DNA-binding protein 1 antibody
- DDB 1 antibody
see all
画像
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Western blot - Anti-DDB1 antibody (ab9194)All lanes : Anti-DDB1 antibody (ab9194) at 1 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 127 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.
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Western blot - Anti-DDB1 antibody (ab9194)All lanes : Anti-DDB1 antibody (ab9194) at 0.01 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NSO (Murine myeloma cells) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 127 kDa
Observed band size: 140,150 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB1 antibody (ab9194)Left panel: Immunohistochemical analysis of paraffin-embedded human cortex tissue labelling DDB1 with ab9194 at 4µg/ml. Heat mediated antigen retrieval with citrate buffer pH 6, HRP-staining.
Right panel: Immunohistochemical analysis of paraffin-embedded human cortex tissue with no primary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab9194 は 12 報の論文で使用されています。
- Titelbaum M et al. Ezh2 harnesses the intranuclear actin cytoskeleton to remodel chromatin in differentiating Th cells. iScience 24:103093 (2021). PubMed: 34622148
- Yang C et al. Small molecule NSC1892 targets the CUL4A/4B-DDB1 interactions and causes impairment of CRL4DCAF4 E3 ligases to inhibit colorectal cancer cell growth. Int J Biol Sci 16:1059-1070 (2020). PubMed: 32140073
- Tong X et al. DDB1 E3 ligase controls dietary fructose-induced ChREBPa stabilization and liver steatosis via CRY1. Metabolism 107:154222 (2020). PubMed: 32246987
- Sun H et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). PubMed: 30779731
- Pines A et al. TRiC controls transcription resumption after UV damage by regulating Cockayne syndrome protein A. Nat Commun 9:1040 (2018). WB . PubMed: 29531219