Anti-DCTN1/p150-glued 抗体 [EPR26464-28] (ab302629)
![Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-489471-anti-dctn1p150glued-antibody-epr2646428-western-blot-hela-2.jpg "Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26464-28] to DCTN1/p150-glued
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IP
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DCTN1/p150-glued antibody [EPR26464-28]
DCTN1/p150-glued 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26464-28] to DCTN1/p150-glued -
由来種
Rabbit -
特異性
IHC application not suitable for human and rat species.
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アプリケーション
適用あり: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IPmore details -
種交差性
交差種: Mouse, Human
非交差種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa transfected with siRNA specifically targeting DCTN1 and a scrambled siRNA control, Neuro-2a, and NIH/3T3 whole cell lysates, mouse brain and cerecbellum tissue lysates. IHC-P: Mouse cerebrum, spinal cord, and dorsal root ganglion FFPE tissue sections. IHC-Fr: Mouse spinal cord fresh frozen tissue section. ICC/IF: HeLa and Neuro-2a cell lines. Flow Cyt (Intra): HeLa and Neuro-2a cell lines. IP: HeLa and Neuro-2a whole cell lysates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26464-28 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302629の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 141 kDa).
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IHC-Fr |
1/100.
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| ICC/IF |
1/50.
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| Flow Cyt (Intra) |
1/50.
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| IP |
1/30.
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 141 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IHC-Fr
1/100. |
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ICC/IF
1/50. |
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Flow Cyt (Intra)
1/50. |
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IP
1/30. |
ターゲット情報
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機能
Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. -
組織特異性
Brain. -
関連疾患
Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B) [MIM:607641]; also known as progressive lower motor neuron disease (PLMND). HMN7B is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
Defects in DCTN1 are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
Defects in DCTN1 are the cause of Perry syndrome (PERRYS) [MIM:168605]; also called parkinsonism with alveolar hypoventilation and mental depression. Perry syndrome is a neuropsychiatric disorder characterized by mental depression not responsive to antidepressant drugs or electroconvulsive therapy, sleep disturbances, exhaustion and marked weight loss. Parkinsonism develops later and respiratory failure occurred terminally. -
配列類似性
Belongs to the dynactin 150 kDa subunit family.
Contains 1 CAP-Gly domain. -
翻訳後修飾
Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 1639 Human
- Entrez Gene: 13191 Mouse
- Omim: 601143 Human
- SwissProt: Q14203 Human
- SwissProt: O08788 Mouse
- Unigene: 516111 Human
- Unigene: 6919 Mouse
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別名
- 150 kDa dynein associated polypeptide antibody
- 150 kDa dynein-associated polypeptide antibody
- DAP 150 antibody
see all
画像
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Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)All lanes : Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse cerecbellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
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![Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-489472-anti-dctn1p150glued-antibody-epr2646428-western-blot-hela.jpg)
Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)All lanes : Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate
Lane 2 : Hela transfected with siRNA specifically targeting DCTN1, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 141 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 114 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-8-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse cerebrum (PMID:17122035). The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-9-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-mouse-dorsal-root-ganglia.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Immunohistochemical analysis of paraffin-embedded mouse dorsal root ga tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse dorsal root ganglion (PMID:23874158). The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-10-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-mouse-spinal-cord.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse spinal cord (PMID:23408943, 17122035 ). The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-11-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling DCTN1/p150-glued with ab302629 at 1/1000 (0.539 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse liver (PMID:1836789). The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
-
![Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-6-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-frozen-mouse-spinal-cord.jpg)
Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spinal cord (fresh) tissue labeling DCTN1/p150-glued with ab302629 at 1/100 (1.078 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on mouse spinal cord is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
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![Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-7-anti-dctn1p150glued-antibody-epr2646428-immunohistochemistry-frozen-mouse-liver.jpg)
Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling DCTN1/p150-glued with ab302629 at 1/100 (1.078 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Negative control: no staining on mouse liver (PMID:1836789) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-4-anti-dctn1p150glued-antibody-epr2646428-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling DCTN1/p150-glued with ab302629 at 1/50 (10.78 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/ml dilution) (Green). Confocal image showing mainly cytoplasmic staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2µg/ml) dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-5-anti-dctn1p150glued-antibody-epr2646428-immunocytochemistry-neuro2a.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling DCTN1/p150-glued with ab302629 at 1/50 (10.78 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2µg/ml) dilution.
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![Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-2-anti-dctn1p150glued-antibody-epr2646428-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling DCTN1/p150-glued with ab302629 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-3-anti-dctn1p150glued-antibody-epr2646428-flow-cytometry-neuro2a.jpg)
Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling DCTN1/p150-glued with ab302629 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-12-anti-dctn1p150glued-antibody-epr2646428-immunoprecipitation-hela.jpg)
Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)DCTN1/p150-glued was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg with ab302629 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302629 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 µg
Lane 2: ab302629 IP in HeLa whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab302629 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-13-anti-dctn1p150glued-antibody-epr2646428-immunoprecipitation-neuro2a.jpg)
Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)DCTN1/p150-glued was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 µg with ab302629 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302629 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 µg
Lane 2: ab302629 IP in Neuro-2a whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab302629 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)](https://www.abcam.co.jp/ps/products/302/ab302629/Images/AB302629-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab302629 は論文での使用が確認できていません。