Anti-Dcr 2 / Dicer 2 抗体 (ab4732)
Key features and details
- Rabbit polyclonal to Dcr 2 / Dicer 2
- Suitable for: WB, ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG
製品の概要
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製品名
Anti-Dcr 2 / Dicer 2 antibody -
製品の詳細
Rabbit polyclonal to Dcr 2 / Dicer 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Drosophila melanogaster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4732の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 188 kDa.
Other (lower) bands are also seen in the blot (see image). We are unsure as to the identity of these - they may be cleavage products of Dcr2, or unspecific bands. These bands are all, however, blocked with the immunising peptide. |
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 188 kDa. Other (lower) bands are also seen in the blot (see image). We are unsure as to the identity of these - they may be cleavage products of Dcr2, or unspecific bands. These bands are all, however, blocked with the immunising peptide. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified. The Saccharomyces cerevisiae cell cycle regulator genes DCR2 and DCR1 appear to be involved in the initiation of DNA replication. It has been predicted that DCR2 might encode a protein with phosphoesterase activity. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 36993 Drosophila melanogaster
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別名
- Dcr2 antibody
- Dicer antibody
- Dicer2 antibody
画像
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Western blot - Anti-Dcr 2 / Dicer 2 antibody (ab4732)Anti-Dcr 2 / Dicer 2 antibody (ab4732) at 1 µg/ml + Drosophila embryo nuclear extract (from D. melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 188 kDa
Observed band size: 171,200 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab4732 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab4732 は 13 報の論文で使用されています。
- Rauskolb C et al. Analysis of the Drosophila Ajuba LIM protein defines functions for distinct LIM domains. PLoS One 17:e0269208 (2022). PubMed: 35969522
- Romano G et al. TDP-43 prevents retrotransposon activation in the Drosophila motor system through regulation of Dicer-2 activity. BMC Biol 18:82 (2020). PubMed: 32620127
- Ge DT et al. The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria. Mol Cell 74:982-995.e6 (2019). PubMed: 31076285
- Harrington AW et al. Drosophila melanogaster retrotransposon and inverted repeat-derived endogenous siRNAs are differentially processed in distinct cellular locations. BMC Genomics 18:304 (2017). PubMed: 28415970
- Sinha NK & Bass BL Overexpression and purification of Dicer and accessory proteins for biochemical and structural studies. Methods 126:54-65 (2017). PubMed: 28723582