Anti-DCK 抗体 (ab186128)
Key features and details
- Rabbit polyclonal to DCK
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DCK antibody
DCK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DCK -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide within Human DCK aa 200 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_000779.1).
Database link: P27707 -
ポジティブ･コントロール
- WB: HeLa, 293T, Jurkat, TCMK-1 and NIH 3T3 whole cell lysates. IP: HeLa cell lysate. IHC-P: Human ovarian carcinoma and mouse teratoma tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab186128 was affinity purified using an epitope specific to DCK immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186128の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/1000.
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| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 31 kDa.
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| 特記事項 |
|---|
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IHC-P
1/1000. |
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 31 kDa. |
ターゲット情報
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機能
Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. -
配列類似性
Belongs to the DCK/DGK family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 422648 Chicken
- Entrez Gene: 461273 Chimpanzee
- Entrez Gene: 482188 Dog
- Entrez Gene: 1633 Human
- Entrez Gene: 13178 Mouse
- Entrez Gene: 100517354 Pig
- Entrez Gene: 79127 Rat
- Entrez Gene: 705040 Rhesus monkey
see all -
別名
- dCK antibody
- DCK protein antibody
- DCK_HUMAN antibody
see all
画像
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Western blot - Anti-DCK antibody (ab186128)All lanes : Anti-DCK antibody (ab186128) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-DCK antibody (ab186128)Detection of DCK in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186128 at 6 µg per reaction for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody (ab186128)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling DCK with ab186128 at a 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCK antibody (ab186128)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse teratoma tissue labeling DCK with ab186128 at a 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab186128 は 1 報の論文で使用されています。
- Ma YT et al. Use of a genome-wide haploid genetic screen to identify treatment predicting factors: a proof-of-principle study in pancreatic cancer. Oncotarget 8:63635-63645 (2017). PubMed: 28969017