Anti-DCAF17 抗体 (ab185324)
Key features and details
- Rabbit polyclonal to DCAF17
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DCAF17 antibody -
製品の詳細
Rabbit polyclonal to DCAF17 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human DCAF17 aa 300-450.
Database link: Q5H9S7 -
ポジティブ･コントロール
- RT-4 and U-251 MG sp cell lysates; Human tonsil tissue lysate; Human cerebral cortex tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185324の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa.
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| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa. |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Interacts with DDB1, CUL4A and CUL4B. -
細胞内局在
Membrane; Multi-pass membrane protein. Nucleus; nucleolus. -
参照データベース
- Entrez Gene: 80067 Human
- Entrez Gene: 75763 Mouse
- Entrez Gene: 499807 Rat
- Omim: 612515 Human
- SwissProt: Q5H9S7 Human
- SwissProt: Q3TUL7 Mouse
- SwissProt: B1H299 Rat
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別名
- C2orf37 antibody
- Chromosome 2 open reading frame 37 antibody
- DCAF17 antibody
- DDB1- and CUL4-associated factor 17 antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAF17 antibody (ab185324)
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue, labeling DCAF17 with ab185324 at 1/1000 dilution.
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Western blot - Anti-DCAF17 antibody (ab185324)All lanes : Anti-DCAF17 antibody (ab185324) at 1/500 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG sp cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 59 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185324 は論文での使用が確認できていません。