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リコンビナントRabMAb

Anti-DC-SIGN 抗体 [EPR22395-52] - BSA and Azide free (ab245200)

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Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)
  • Flow Cytometry - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)
  • Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22395-52] to DC-SIGN - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free
    DC-SIGN 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22395-52] to DC-SIGN - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, Flow Cytmore details
    適用なし: IHC-P,IP or WB

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: THP-1 cells. Flow: THP-1 cells.

  • 特記事項

    ab245200 is the carrier-free version of ab245189.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245200の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P,IP or WB.

ターゲット情報

  • 機能

    Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
    On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.

  • 組織特異性

    Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.

  • 配列類似性

    Contains 1 C-type lectin domain.

  • ドメイン

    The tandem repeat domain, also called neck domain, mediates oligomerization.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C type lectin domain family 4 member L antibody
      • C-type lectin domain family 4 member L antibody
      • CD 209 antibody
      • CD209 antibody
      • CD209 antigen antibody
      • CD209 antigen-like protein A antibody
      • CD209 molecule antibody
      • CD209_HUMAN antibody
      • Cd209a antibody
      • CDSIGN antibody
      • CIRE antibody
      • CLEC4L antibody
      • DC SIGN antibody
      • DC SIGN1 antibody
      • DC-SIGN antibody
      • DC-SIGN1 antibody
      • DCSIGN antibody
      • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
      • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
      • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
      • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
      • Dengue fever, protection against, included antibody
      • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
      • HIV GP120 Binding Protein antibody
      • MGC129965 antibody
      • MGC130443 antibody
      • SIGN-R1 antibody
      • SIGNR5 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)
      Immunocytochemistry/ Immunofluorescence - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling DC-SIGN using ab245189 at 1/100 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in THP-1 cells treated with 10 ng/ml PMA for 18h, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN expression is induced by PMA plus IL4 in THP-1 (PMID: 15070901; PMID: 22675249). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to stain tubulin (red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245189).

    • Flow Cytometry - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)
      Flow Cytometry - Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)

      Flow cytometric analysis of 4% paraformaldehyde-fixed THP-1 (human monocytic leukemia cell line) cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN is labeled with ab245189 at 1/60 dilution in treated (red) and untreated cells (green) as compared to a Rabbit monoclonal IgG (ab172730, black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody, blue).  A Goat anti rabbit IgG (Dylight ® 488, ab98462) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells. DC-SIGN expression is induced by PMA plus IL4 in THP-1 (PMID: 15070901; PMID: 22675249).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245189).

    • Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)
      Anti-DC-SIGN antibody [EPR22395-52] - BSA and Azide free (ab245200)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245200 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245200 は論文での使用が確認できていません。

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