Anti-DBC-1 抗体 (ab70239)
Key features and details
- Rabbit polyclonal to DBC-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DBC-1 antibody
DBC-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DBC-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human DBC-1 aa 1-50 (N terminal).
Database link: Q8N163 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70239の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/10000 - 1/20000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
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| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/10000 - 1/20000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. -
組織特異性
Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 464047 Chimpanzee
- Entrez Gene: 507114 Cow
- Entrez Gene: 486122 Dog
- Entrez Gene: 101135799 Gorilla
- Entrez Gene: 57805 Human
- Entrez Gene: 100173017 Orangutan
- Entrez Gene: 100155718 Pig
- Entrez Gene: 716288 Rhesus monkey
see all -
別名
- CCAR2 antibody
- Cell cycle and apoptosis regulator 2 antibody
- Cell division cycle and apoptosis regulator protein 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody (ab70239)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DBC-1 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-DBC-1 antibody (ab70239)All lanes : Anti-DBC-1 antibody (ab70239) at 1/10000 dilution
Lane 1 : HeLa cell nuclear extract at 15 µg
Lane 2 : HeLa cell nuclear extract at 45 µg
Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70239 は 3 報の論文で使用されています。
- Iqbal IK et al. Hydrogen sulfide-induced GAPDH sulfhydration disrupts the CCAR2-SIRT1 interaction to initiate autophagy. Autophagy 17:3511-3529 (2021). PubMed: 33459133
- Han C et al. O-GlcNAcylation of SIRT1 enhances its deacetylase activity and promotes cytoprotection under stress. Nat Commun 8:1491 (2017). PubMed: 29133780
- Shao D et al. A redox-resistant sirtuin-1 mutant protects against hepatic metabolic and oxidant stress. J Biol Chem 289:7293-306 (2014). PubMed: 24451382