Anti-DAZL 抗体 (ab34139)
Key features and details
- Rabbit polyclonal to DAZL
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DAZL antibody
DAZL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DAZL -
由来種
Rabbit -
特異性
From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Cow, Human, Chimpanzee, Common marmoset
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免疫原
Synthetic peptide corresponding to Mouse DAZL aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab27733) -
ポジティブ･コントロール
- ICC/IF: mES cells. IHC-P: Mouse, human and common marmoset testis tissue. WB: Human and mouse testis tissue lysate, GST tagged Recombinant Human DAZL protein (ab112279).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34139の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (6) |
Use a concentration of 0.5 - 5 µg/ml.
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| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 0.5 - 5 µg/ml. |
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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機能
RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts. -
組織特異性
Testis specific. -
配列類似性
Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain. -
ドメイン
The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2. -
細胞内局在
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward. - Information by UniProt
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参照データベース
- Entrez Gene: 460209 Chimpanzee
- Entrez Gene: 530116 Cow
- Entrez Gene: 1618 Human
- Entrez Gene: 13164 Mouse
- Omim: 601486 Human
- SwissProt: Q92904 Human
- SwissProt: Q64368 Mouse
- Unigene: 131179 Human
see all -
別名
- DAZ homolog antibody
- DAZ like autosomal antibody
- DAZ-like autosomal antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody (ab34139)
ab34139 staining DAZL in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab34139 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody (ab34139)IHC image of DAZL staining in a section of formalin-fixed paraffin-embedded normal mouse testis performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab34139, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody (ab34139)This image is courtesy of an Abreview submitted by Dr Sharon Sneddonab34139 at 1/100 staining human blastocyst cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor ® 488 conjugated secondary antibody was used.
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Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody (ab34139)The image, submitted as part of an Abreview, shows DAZL staining (green) of a human blastocyst. The antibody stains the cytoplasm of cells of the inner cell mass and trophoblast. DAZL is known to be located to the cytoplasm and DAZL transcripts have previously been discovered in the inner cell mass and trophblast (see PMID: 15879466). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody (ab34139)The image shows staining of human testis tissue. The cytoplasm of Leydig cells and other cells in the seminiferous tubules stained positive. The antigen retrieval techniques used were Citrate at pH6 or Tris EDTA at pH9. -
Western blot - Anti-DAZL antibody (ab34139)All lanes : Anti-DAZL antibody (ab34139) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 70 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody (ab34139)This image is taken from an abreview by Zachary Yu-Ching Lin.Ab34139 staining DAZL on juvenile marmoset testes by immunohistochemistry. The tissue was paraformaldehyde fixed and blocked in 1% milk for 1 hour at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 9 hours at 4°C in 5% milk. An Alexa Fluor 555 conjugated goat anti-rabbit antibody was used as the secondary. Nuclear staining shown in blue with DAPI. -
Western blot - Anti-DAZL antibody (ab34139)Anti-DAZL antibody (ab34139) at 1 µg/ml + GST tagged Recombinant Human DAZL protein (ab112279) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Exposure time: 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (77)
ab34139 は 77 報の論文で使用されています。
- Shimada R et al. STRA8-RB interaction is required for timely entry of meiosis in mouse female germ cells. Nat Commun 14:6443 (2023). ICC/IF ; Mouse . PubMed: 37880249
- Seita Y et al. Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells. Elife 12:N/A (2023). PubMed: 36719274
- Reifarth L et al. Detection of spermatogonial stem cells in testicular tissue of dogs with chronic asymptomatic orchitis. Front Vet Sci 10:1205064 (2023). PubMed: 37396999
- Ghasemi D et al. Differentiation of human endometrial stem cells encapsulated in alginate hydrogel into oocyte-like cells. Bioimpacts 13:229-240 (2023). PubMed: 37431484
- Irie N et al. DMRT1 regulates human germline commitment. Nat Cell Biol 25:1439-1452 (2023). PubMed: 37709822