Name: Anti-DAZAP1 antibody [EPR14400-60]
Brand: RabMAb
SKU: ab184183
Rating: 4 (3 reviews)

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Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14400-60] to DAZAP1
Suitable for: WB, IP
Reacts with: Human

Carrier Free

製品名

Anti-DAZAP1 antibody [EPR14400-60]
DAZAP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR14400-60] to DAZAP1
由来種

Rabbit
アプリケーション

適用あり: WB, IPmore details
種交差性

交差種: Human
交差が予測される動物種: Rat
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Jurkat, HeLa, HepG2 and 293T cell lysates.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR14400-60
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-DAZAP1 antibody [EPR14400-60] - BSA and Azide free (ab250737)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human DAZAP1 knockout HEK-293T cell line (ab266469)
KO cell lysates
- Human DAZAP1 knockout HEK-293T cell lysate (ab257911)
- Human DAZAP1 knockout HEK-293T cell lysate (ab257912)
Positive Controls

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab184183の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(1)	1/10000 - 1/50000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP	(1)	1/40 - 1/60.

特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP 1/40 - 1/60.

機能

RNA-binding protein, which may be required during spermatogenesis.
組織特異性

Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
配列類似性

Contains 2 RRM (RNA recognition motif) domains.
翻訳後修飾

Phosphorylated upon DNA damage, probably by ATM or ATR.
細胞内局在

Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
Target information above from: UniProt accession Q96EP5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 26528 Human
- Entrez Gene: 362836 Rat
- Omim: 607430 Human
- SwissProt: Q96EP5 Human
- Unigene: 222510 Human
別名
- DAZ associated protein 1 antibody
- DAZ-associated protein 1 antibody
- Dazap1 antibody
- DAZP1_HUMAN antibody
- Deleted in azoospermia associated protein 1 antibody
- Deleted in azoospermia-associated protein 1 antibody
see all

Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAZAP1 CRISPR/Cas9 edited HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Lanes 1-4: Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line ab266470 (CRISPR/Cas9 edited cell lysate ab257912) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAZAP1 CRISPR/Cas9 edited HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Lanes 1-4: Merged signal (red and green). Green - ab184183 observed at 43 kDa. Red - loading control ab8245 observed at 36 kDa.

ab184183 Anti-DAZAP1 antibody [EPR14400-60] was shown to specifically react with DAZAP1 in wild-type HEK293T cells. The band observed in CRISPR/Cas9 edited cell line ab266469 (CRISPR/Cas9 edited cell lysate ab257911) lane below 43 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and DAZAP1 CRISPR/Cas9 edited samples were subjected to SDS-PAGE. ab184183 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

All lanes : Anti-DAZAP1 antibody [EPR14400-60] (ab184183) at 1/10000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa
Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

Western blot analysis of DAZAP1 in Jurkat cell lysate immunoprecipitated using ab184183 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Jurkat lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Anti-DAZAP1 antibody [EPR14400-60] (ab184183)

Western blot protocols
Immunoprecipitation protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab184183 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184183 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (B-cell lymphocyte whole cell lysate)

Total protein in input

300 µg

Immuno-precipitation step

Protein A

Specification

B-cell lymphocyte whole cell lysate

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 12 2019

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (fibroblast)

Permeabilization

Yes - 0.3% Triton X-100 in blocking buffer

Specification

fibroblast

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 27 2018

Application

Western blot

Sample

Human Cell lysate - whole cell (Lymphocytes)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris)

Loading amount

30 µg

Specification

Lymphocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 21 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A

Immunoprecipitation abreview for Anti-DAZAP1 antibody [EPR14400-60]

Immunocytochemistry/ Immunofluorescence abreview for Anti-DAZAP1 antibody [EPR14400-60]

Western blot abreview for Anti-DAZAP1 antibody [EPR14400-60]