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リコンビナントRabMAb

Anti-DARPP32 抗体 [EP721Y] (ab40802)

Submit a review Submit a question References (3)
Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)
  • Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (ab40802)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)
  • Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (ab40802)
  • Anti-DARPP32 antibody [EP721Y] (ab40802)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP721Y] to DARPP32
  • Suitable for: ICC/IF, WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Carrier Free

製品の概要

  • 製品名

    Anti-DARPP32 antibody [EP721Y]
    DARPP32 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP721Y] to DARPP32

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human brain lysate and human gastric adenocarcinoma. SH-SY5Y (human neuroblastoma) and human fetal forebrain tissue lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab40802の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50 - 1/100.
WB
1/5000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).

Unpurified format: 1/5,000 - 1/20,000
IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50.
特記事項
ICC/IF
1/50 - 1/100.
WB
1/5000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).

Unpurified format: 1/5,000 - 1/20,000
IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50.

ターゲット情報

  • 機能

    Inhibitor of protein-phosphatase 1.

  • 配列類似性

    Belongs to the protein phosphatase inhibitor 1 family.

  • 翻訳後修飾

    Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
    Phosphorylation of Thr-34 is required for activity.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DARPP 32 antibody
      • DARPP-32 antibody
      • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
      • Dopamine and cAMP regulated neuronal phosphoprotein antibody
      • Dopamine and cAMP regulated phosphoprotein antibody
      • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
      • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
      • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
      • FLJ20940 antibody
      • IPPD antibody
      • Neuronal phosphoprotein DARPP 32 antibody
      • PPP1R1B antibody
      • PPR1B_HUMAN antibody
      • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
      • Protein phosphatase 1 regulatory subunit 1B antibody
      see all

    画像

    • Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802)
      Western blot - Anti-DARPP32 antibody [EP721Y] (ab40802)
      Anti-DARPP32 antibody [EP721Y] (ab40802) at 1/50000 dilution + Human brain tissue lysates. at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 32 kDa
      Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)

      ab40802 staining DARPP32 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

      Negative control 1: PBS in place of primary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (ab40802)
      Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP721Y] (ab40802)

      ab40802 staining DARPP32 in SH-SY5Y (human neuroblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab40802 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP721Y] (ab40802)
      Immunohistochemical analysis human gastric adenocarcinoma using ab40802. (dilution 1:250)
    • Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (ab40802)
      Immunoprecipitation - Anti-DARPP32 antibody [EP721Y] (ab40802)

      ab40802 immunoprecipitating DARPP32. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

      Lane 1: Human fetal forebrain whole cell lysate (10ug)
      Lane 2: Human fetal forebrain whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40802 in Human fetal forebrain whole cell lysate

    • Anti-DARPP32 antibody [EP721Y] (ab40802)
      Anti-DARPP32 antibody [EP721Y] (ab40802)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (3)

    ab40802 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab40802 は 3 報の論文で使用されています。

    • Sanders B  et al. Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants. Nat Commun 13:27 (2022). PubMed: 35031607
    • Lange J  et al. Mislocalization of Nucleocytoplasmic Transport Proteins in Human Huntington's Disease PSC-Derived Striatal Neurons. Front Cell Neurosci 15:742763 (2021). PubMed: 34658796
    • André EM  et al. A Combinatorial Cell and Drug Delivery Strategy for Huntington's Disease Using Pharmacologically Active Microcarriers and RNAi Neuronally-Committed Mesenchymal Stromal Cells. Pharmaceutics 11:N/A (2019). PubMed: 31614758

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