Name: Anti-DARPP32 antibody [EP720Y]
Brand: RabMAb
SKU: ab40801
Rating: 4 (11 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP720Y] to DARPP32
Suitable for: ICC/IF, WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 750 Carrier Free

製品名

Anti-DARPP32 antibody [EP720Y]
DARPP32 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP720Y] to DARPP32
由来種

Rabbit
アプリケーション

適用あり: ICC/IF, WB, IHC-P, IPmore details
種交差性

交差種: Mouse, Rat, Human
交差が予測される動物種: Pig
免疫原

Synthetic peptide within Human DARPP32 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UD71
(Peptide available as ab189245)
ポジティブ･コントロール
- IHC-P: Human breast adenocarcinoma tissue and colon tissue. Mouse and rat cerebral cortex tissue. ICC/IF: Mouse brain tissue. WB: Rat hippocampus and brain tissue lysate. Mouse cerebral cortex tissue lysate. Human fetal brain lysate. IP: Rat brain whole cell lysate. IHC-Wm: Mouse brain tissue. IHC-Fr: Pig brain tissue.
特記事項
Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) is a protein that in humans is encoded by the PPP1R1B gene. This gene, also known as DARPP-32, has a role as a dopamine- and cyclic AMP-regulated phosphoprotein. As such PPP1R1B affects dopamine glutamate and adenosine; and potentially has a role in conditions such as schizophrenia and Parkinson's disease.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP720Y
アイソタイプ

IgG
研究分野
- Neuroscience
- Processes

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Rat brain cerebrum tissue lysate - total protein (ab4033)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab40801の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF	(2)	1/50 - 1/100.
WB	(3)	1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). For unpurified use at 1/5000 - 1/50 000.
IHC-P		1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IP		1/20. For unpurified use at 1/50.

特記事項
ICC/IF 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). For unpurified use at 1/5000 - 1/50 000.
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
IP 1/20. For unpurified use at 1/50.

機能

Inhibitor of protein-phosphatase 1.
配列類似性

Belongs to the protein phosphatase inhibitor 1 family.
翻訳後修飾

Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
細胞内局在

Cytoplasm.
Target information above from: UniProt accession Q9UD71 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 84152 Human
- Entrez Gene: 19049 Mouse
- Entrez Gene: 360616 Rat
- Omim: 604399 Human
- SwissProt: Q9UD71 Human
- SwissProt: Q60829 Mouse
- SwissProt: Q6J4I0 Rat
- Unigene: 286192 Human
- Unigene: 45372 Mouse
- Unigene: 70366 Rat
see all
別名
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein antibody
- Dopamine and cAMP regulated phosphoprotein antibody
- Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
- Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
- Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
- FLJ20940 antibody
- IPPD antibody
- Neuronal phosphoprotein DARPP 32 antibody
- PPP1R1B antibody
- PPR1B_HUMAN antibody
- Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
- Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
- Protein phosphatase 1 regulatory subunit 1B antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunohistochemical analysis of paraffin-embedded human colon sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-DARPP32 antibody [EP720Y] (ab40801)

All lanes : Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (purified)

Lane 1 : Rat hippocampus tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse cerebral cortex tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Blocking/Diluting buffer 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunocytochemistry/Immunofluorescence analysis of mouse brain tissue labelling DARPP32 with purified ab40801 at 1/100 (3.4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was performed with Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody.

Secondary Only Control: PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunoprecipitation - Anti-DARPP32 antibody [EP720Y] (ab40801)

ab40801 at 1/20 immunoprecipitating DARPP32 in rat brain whole cell lysate observed at 32 KDa (lanes 1 and 2).

Lane 1: Rat brain whole cell lysate 10μg.

Lane 2: ab40801 IP in rat brain whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40801 in rat brain whole cell lysate.

For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection (1/1000).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-DARPP32 antibody [EP720Y] (ab40801)

Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/1000 dilution (purified) + Human fetal brain lysate at 15 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa

Blocking/Diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunohistochemical analysis of human breast adenocarcinoma sections labelling DARPP32 with unpurified ab40801 at a dilution of 1/50.
Western blot - Anti-DARPP32 antibody [EP720Y] (ab40801)

Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (unpurified) + Rat brain lysate at 10 µg

Predicted band size: 32 kDa
Observed band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARPP32 antibody [EP720Y] (ab40801)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Anti-DARPP32 antibody [EP720Y] (ab40801)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab40801 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab40801 は 110 報の論文で使用されています。

Morelli KH et al. An RNA-targeting CRISPR-Cas13d system alleviates disease-related phenotypes in Huntington's disease models. Nat Neurosci 26:27-38 (2023). PubMed: 36510111
Gangwani MR et al. Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors. Cell Rep 42:111953 (2023). PubMed: 36640336
Wang Y et al. Water-Reaching Platform for Longitudinal Assessment of Cortical Activity and Fine Motor Coordination Defects in a Huntington Disease Mouse Model. eNeuro 10:N/A (2023). PubMed: 36596592
Gutiérrez-Garcia R et al. G3BP1-dependent mechanism suppressing protein aggregation in Huntington's models and its demise upon stress granule assembly. Hum Mol Genet 32:1607-1621 (2023). PubMed: 36611004
Wu GH et al. CryoET reveals organelle phenotypes in huntington disease patient iPSC-derived and mouse primary neurons. Nat Commun 14:692 (2023). PubMed: 36754966

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-10 of 12 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Differentiated NPC)

Permeabilization

Yes - Triton X-100

Specification

Differentiated NPC

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 22 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Brain (Hippocampus/entorhinal))

Permeabilization

Yes - Citrate Buffer, Triton X-100

Specification

Brain (Hippocampus/entorhinal)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

SRA. PATRICIA VILLANUEVA ANGUITA

Verified customer

投稿 Nov 17 2021

Application

Immunohistochemistry free floating

Sample

Mouse Tissue sections (Brain, 45 µm VIBRATOME sections)

Specification

Brain, 45 µm VIBRATOME sections

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 07 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (human)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Treatment

alpha synuclein

Specification

human

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Sehee Oh

Verified customer

投稿 Jul 13 2016

Application

Flow Cytometry

Sample

Human Cell (Differentiated hNSCs)

Permeabilization

Yes - 0.25% Triton X-100 in DPBS

Gating Strategy

Undifferentiated Stem Cells (white)

Specification

Differentiated hNSCs

Preparation

Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS

Fixation

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 18 2015

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Sample

Human Cell (Differentiated hNSCs)

Specification

Differentiated hNSCs

Permeabilization

Yes - 0.25% Triton X-100 in DPBS

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 17 2015

Application

Immunohistochemistry (Frozen sections)

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Sample

Sheep Tissue sections (Brain Median Eminence)

Specification

Brain Median Eminence

Permeabilization

Yes - 0.01% Triton

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. XXXXXX XXXXXX

Verified customer

投稿 Oct 24 2014

Application

IHC - Wholemount

Sample

Zebra finch Tissue (14um brain section)

Specification

14um brain section

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 13 2014

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (12% Tris Glycine)

Sample

Zebra finch Tissue lysate - other (Brain cytoplasmic fraction)

Specification

Brain cytoplasmic fraction

Blocking step

Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 14 2013

Question

Thank you so much for helping me find primary (and secondary) antibodies for IHC on mouse neural progenitors down the lineage of cortex verses striatum. Attached is the paper (2012) that mentions cortex being identified with NCAM (Figure 4 i,j page 481) and Figure 1 says cortical induction efficiency was measured by Pax6, Vimentin, Otx1 and Otx2 expression (Figure 1c, page 478). The second paper I attached (2008) is to help us figure out a good primary/secondary antibody detection of striatal differentiation. It says “Terminal striatal differentiation can be established by the detection ofMAP-2/Ki67[1] postmitotic neurons expressing key striatal markers, such as GABA, GAD67 (GAD1), DARPP32, ARPP21, calbindin, or calretinin.” If you can find anything more efficient and specific for cortex vs. striatum it would be wonderful! Please contact me for any reason and thank you for your time and energy,

Abcam community

Verified customer

Asked on May 23 2012

Answer

Thank you for contacting us.

While there a many targets which express differentially in distinct layers of the neocortex many of these are expressed in the striatum as well. I found these papers to have a number of good targets which seem specific, such as Pax6 or, for striatal markers, DARPP32, CalB or GAD67. However a search through the Allen Mouse Brain Atlas expression data shows similar gene expression of all these markers in adult striatum and cortex. I would emphasize that this is not vetted data.

While any single or combination of these markers may well yield the information which you will be looking for, I feel that the best choices may be to try a few of these targets presented within this literature as you would have reference material to compare with.

Further review of our catalog leads me to suggest use of a DARPP32 antibody such as ab40801 alongside use of a Vementin antibody such as ab8978. Alternatively use of PAX6 ab5790 alongside GAD67, ab26116, may yield good results.

Each of these pairs will work in double staining as they are comprised of a mouse and a rabbit derived antibody. Further each product has been used in numerous publications and is well characterized by these, Abreviews and scientific support on the datasheet. Each is guaranteed to work in IHC-P as well as a number of other applications and would therefore be covered by our Abpromise guarantee:https://www.abcam.com/abpromise.

I have added links to each of these products below for your review:

ab40801;https://www.abcam.com/DARPP32-antibody-EP720Y-ab40801.html

ab8978;https://www.abcam.com/Vimentin-antibody-RV202-Neural-Stem-Cell-Marker-ab8978.html

ab5790;https://www.abcam.com/PAX6-antibody-Stem-Cell-Marker-ab5790.html

ab26116;https://www.abcam.com/GAD67-antibody-K-87-Neuronal-Marker-ab26116.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Abcam Scientific Support

Answered on May 23 2012

1-10 of 12 Abreviews or Q&A

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (110)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-DARPP32 antibody [EP720Y]

Immunohistochemistry (Frozen sections) abreview for Anti-DARPP32 antibody [EP720Y]

Immunohistochemistry free floating abreview for Anti-DARPP32 antibody [EP720Y]

Western blot abreview for Anti-DARPP32 antibody [EP720Y]

Flow Cytometry abreview for Anti-DARPP32 antibody [EP720Y]

Immunocytochemistry/ Immunofluorescence abreview for Anti-DARPP32 antibody [EP720Y]

Immunohistochemistry (Frozen sections) abreview for Anti-DARPP32 antibody [EP720Y]

IHC - Wholemount abreview for Anti-DARPP32 antibody [EP720Y]

Western blot abreview for Anti-DARPP32 antibody [EP720Y]