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リコンビナントRabMAb

Anti-DAP12 抗体 [EPR23949-37] - BSA and Azide free (Detector) (ab290681)

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Sandwich ELISA - Anti-DAP12 antibody [EPR23949-37] - BSA and Azide free (Detector) (ab290681)
  • Anti-DAP12 antibody [EPR23949-37] - BSA and Azide free (Detector) (ab290681)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23949-37] to DAP12 - BSA and Azide free (Detector)
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-DAP12 antibody [EPR23949-37] - BSA and Azide free (Detector)
    DAP12 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23949-37] to DAP12 - BSA and Azide free (Detector)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab290681 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human DAP12. The recommended pair for sandwich ELISA is:
         Capture: ab290680 , Human DAP12 Capture Antibody (unconjugated)
         Detector: ab290681 , Human DAP12 Detector Antibody (unconjugated)
    The reference range value is 31-2000 pg/mL.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.2
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23949-37

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290681の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR5173-40] to DAP12 - BSA and Azide free (Capture) (ab290680).
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR5173-40] to DAP12 - BSA and Azide free (Capture) (ab290680).

ターゲット情報

  • 機能

    Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.

  • 組織特異性

    Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.

  • 関連疾患

    Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.

  • 配列類似性

    Belongs to the TYROBP family.

  • 翻訳後修飾

    Tyrosine phosphorylated.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DAP 12 antibody
      • DAP12 antibody
      • DNAX activation protein 12 antibody
      • DNAX-activation protein 12 antibody
      • KAR-associated protein antibody
      • KARAP antibody
      • Killer activating receptor associated protein antibody
      • Killer-activating receptor-associated protein antibody
      • PLOSL antibody
      • TYOBP_HUMAN antibody
      • TYRO protein tyrosine kinase binding protein antibody
      • TYRO protein tyrosine kinase-binding protein antibody
      • TYROBP antibody
      see all

    画像

    • Sandwich ELISA - Anti-DAP12 antibody [EPR23949-37] - BSA and Azide free (Detector) (ab290681)
      Sandwich ELISA - Anti-DAP12 antibody [EPR23949-37] - BSA and Azide free (Detector) (ab290681)
      Sandwich ELISA of ab290705 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
    • Anti-DAP12 antibody [EPR23949-37] - BSA and Azide free (Detector) (ab290681)
      Anti-DAP12 antibody [EPR23949-37] - BSA and Azide free (Detector) (ab290681)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290681 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290681 は論文での使用が確認できていません。

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