Anti-DAD1 抗体 (ab23836)
Key features and details
- Rabbit polyclonal to DAD1
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DAD1 antibody -
製品の詳細
Rabbit polyclonal to DAD1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a 14 amino acid sequence within the C terminal region of DAD 1 (Human).
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ポジティブ・コントロール
- HepG2 cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified by affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23836の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa).
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ICC |
Use at an assay dependent dilution.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 12 kDa). |
ICC
Use at an assay dependent dilution. |
ターゲット情報
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機能
Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. -
配列類似性
Belongs to the DAD/OST2 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1603 Human
- GenBank: NP_001335.1 Human
- Omim: 600243 Human
- SwissProt: P61803 Human
- Unigene: 82890 Human
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別名
- DAD 1 antibody
- DAD-1 antibody
- dad1 antibody
see all
画像
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Lane 1 : Anti-DAD1 antibody (ab23836) at 0.5 µg/ml
Lane 2 : Anti-DAD1 antibody (ab23836) at 1 µg/ml
Lane 3 : Anti-DAD1 antibody (ab23836) at 2 µg/ml
All lanes : HepG2 whole cell lysate
Predicted band size: 12 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
ab23836 staining DAD1 in HepG2 cells by Immunocytochemistry.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab23836 は 2 報の論文で使用されています。
- Ding Y et al. The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyltransferase to acetylate correctly folded polypeptides. J Biol Chem 289:32044-55 (2014). WB . PubMed: 25301944
- Murooka TT et al. CCL5 promotes proliferation of MCF-7 cells through mTOR-dependent mRNA translation. Biochem Biophys Res Commun 387:381-6 (2009). WB ; Human . PubMed: 19607806