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Anti-DAB2IP 抗体 (ab87811)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (23)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunoprecipitation - Anti-DAB2IP antibody (ab87811)
  • Western blot - Anti-DAB2IP antibody (ab87811)

Key features and details

  • Rabbit polyclonal to DAB2IP
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

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Anti-GOLPH3L antibody (ab238876)

関連製品

製品の概要

  • 製品名

    Anti-DAB2IP antibody
    DAB2IP 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to DAB2IP
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IPmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Human DAB2IP aa 850-950.
    Database link: Q5VWQ8

  • ポジティブ・コントロール

    • WB: human HeLa, mouse TCKMK-1, Renca, 4T1 and CT26.WT cells IP: HeLa cells
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    ab87811 was affinity purified using an epitope specific to DAB2IP immobilized on solid support.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab87811の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
IP
Use at 2-5 µg/mg of lysate.
特記事項
WB
1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
IP
Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性

    DAB2IP is a Ras GTPase-activating protein (GAP) that acts as a tumor suppressor gene and is inactivated by methylation in prostate and breast cancers. It also interacts with MAP3K5 to disrupt the association of MAP3K5 with the inhibitory 14-3-3 complex.
  • 細胞内局在

    Cytoplasmic
  • 参照データベース

    • Entrez Gene: 153090 Human
    • Entrez Gene: 69601 Mouse
    • Entrez Gene: 192126 Rat
    • Omim: 609205 Human
    • SwissProt: Q5VWQ8 Human
    • SwissProt: Q3UHC7 Mouse
    • SwissProt: Q6P730 Rat
    • 別名

      • AF9Q34 antibody
      • AIP1 antibody
      • ASK interacting protein antibody
      • ASK interacting protein 1 antibody
      • ASK1 interacting protein 1 antibody
      • DAB2 interacting protein antibody
      • DAB2 interaction protein antibody
      • DAB2P antibody
      • DIP1/2 antibody
      • Disabled homolog 2 interacting protein antibody
      • DOC 2/DAB2 interactive protein antibody
      • FLJ39072 antibody
      • KIAA1743 antibody
      • nGAP like protein antibody
      see all

    画像

    • Immunoprecipitation - Anti-DAB2IP antibody (ab87811)
      Immunoprecipitation - Anti-DAB2IP antibody (ab87811)

      Panel A: Western blot of 0.04µg/ml ab87811 with Lane 1: 50µg, Lane 2: 15g, Lane 3: 5g, HeLa whole cell lysate.

      Panel B: HeLa cells with Lane 1: ab87811, Lane2: ab87811 lot: A302-439A, Lane 3: ab87811 lot: A302-440A, Lane 3: Control IgG

       

    • Western blot - Anti-DAB2IP antibody (ab87811)
      Western blot - Anti-DAB2IP antibody (ab87811)
      All lanes : Anti-DAB2IP antibody (ab87811) at 0.1 µg/ml

      Lane 1 : TCKMK-1 (mouse kidney epithelial cell line) whole cell lysate
      Lane 2 : Renca (murine renal carcinoma cell line) whole cell lysate
      Lane 3 : 4T1 (murine mammary carcinoma cell line) whole cell lysate
      Lane 4 : CT26.WT (mouse colon carcinoma cell line) whole cell lysate

      Predicted band size: 132 kDa

    プロトコール

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (23)

    ab87811 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab87811 は 23 報の論文で使用されています。

    • Huang Q  et al. DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer. iScience 26:107606 (2023). PubMed: 37664607
    • Miller AL  et al. DAB2IP Is a Bifunctional Tumor Suppressor That Regulates Wild-Type RAS and Inflammatory Cascades in KRAS Mutant Colon Cancer. Cancer Res 83:1800-1814 (2023). PubMed: 36939385
    • Ju L  et al. circSLCO1B7 suppresses the malignant progression of hepatocellular carcinoma via the miR-556-3p/DAB2IP axis. Aging (Albany NY) 15:13329-13344 (2023). PubMed: 38015711
    • Yun EJ  et al. Targeting Wnt/β-catenin-mediated upregulation of oncogenic NLGN3 suppresses cancer stem cells in glioblastoma. Cell Death Dis 14:423 (2023). PubMed: 37443071
    • Xiong Z  et al. DAB2IP attenuates chemoresistance of triple-negative breast cancer through sequestration of RAC1 to prevent β-catenin nuclear accumulation. Clin Transl Med 12:e1133 (2022). PubMed: 36536485
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-DAB2IP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (B16F10 mouse melanoma)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    20 µg
    Specification
    B16F10 mouse melanoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 11 2018

    Western blot abreview for Anti-DAB2IP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (WM266 Human melanoma)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    20 µg
    Specification
    WM266 Human melanoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 10 2018

    Question

    Thank you for your answer. I would like to receive another vial of the antibody ab87811, I'll let you know if it works fine.
    How long do you think it will take to receive it?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 17 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry the anti-DAB2IP antibody ab87811 did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xx which you should receive within one or two days.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Jan 17 2012

    Question

    Thank you for your prompt answer. 1) How have you prepared the samples: which lysis buffer have you used, have you denatured them by boilingand how have you applied the Abcam controllysates (HeLa, prostate whole tissue)? (It would be great if you could provide the catalog numbers, lot numbers and/or order numbers/dates for these). Samples preparation (my samples and those from ABCAM): I prepared the loading buffet with 950ul of Laemmli Sample Buffer (BIORAD) and 50ul of Betamercaptoethanol. I mixed 25ug of sample + water (to reach 15ul) + 14ul of loading buffer + 1ul of DTT. I boiled them for 5 minutes at 95°C then loaded them. 2) Could you please confirm the blocking agent used (milk or BSA)? I used Superblock T20 blocking buffer 3) At which dilution and which incubation temperature/time have you applied the primary antibody? dilution of 1:1000, incubation at 4°C overnight 4) Have you run a no-primary control to check the secondary antibody? No because we routinely use this secondary antibody and we never had a problem. I and other colleagues have used the same lot I used in this experiment the following day with another primary antibody and it worked very well. It is part of the ECL Western Blotting Analysis Kit. Once we have received this information and it turns out that the antibodyis not functioning as cited on the product data sheet, we will be pleased to provide afree of charge replacement, acredit note or refund.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 13 2012

    Answer

    Thank you for confirming these details. The details you have kindly provided will provide us with vital information for our monitoring of product quality. Although it is not necessary to add further loading buffer to the lysates you purchased from us, I do not see a reason why it should not work the way you processed the samples. Also, I am pleased to hear that the secondary antibody works fine - that was all we needed to know about it. Thus, reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial. I apologise for the inconvenience and am pleased to offer you a free of charge replacement with a new vial of ab87811. Otherwise, as we currently do not have an alternative Anti-DAB2IP antibody available, I could send you any primary antibody of your choice or issue you a credit note in compensation. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Jan 13 2012

    Question

    Dear sir or madam, I have purchased the antibody ab87811 for DAB2IP which, according to the ABCAM website, should give the following result: ... I tested it in my samples and in the HeLa cell lysate (purchased from ABCAM), and unfortunately these are my results: ... I loaded 25ug of proteins per well, in the HeLa the expected band is missing but there are 4 more in other positions (very different from the ABCAM website), and a similar result was obtained in the prostate whole tissue lysates (from ABCAM). How could you explain these results? Could you send me another antibody for DAB2IP? It must be raised either in mouse or rabbit, anti human. Thank you for your help.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 12 2012

    Answer

    Thank you for taking the time tocontact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this Anti-DAB2IP antibody. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. So far, it seems the bands you detected are indeedrather unspecific. Before deciding though how to proceed with this particular case, I would appreciate if you can confirm some details of the procedure which we alsorequire for our quality controls: 1) How have you prepared the samples: which lysis buffer have you used, have you denatured them by boilingand how have you applied the Abcam controllysates (HeLa, prostate whole tissue)? (It would be great if you could provide the catalog numbers, lot numbers and/or order numbers/dates for these). 2) Could you please confirm the blocking agent used (milk or BSA)? 3) At which dilution and which incubation temperature/time have you applied the primary antibody? 4) Have you run a no-primary control to check the secondary antibody? Once we have received this information and it turns out that the antibodyis not functioning as cited on the product data sheet, we will be pleased to provide afree of charge replacement, acredit note or refund. In order to arrange for the replacement, could you please confirm the following order details?Thank you for your cooperation. I look forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Jan 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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