Anti-Cytosolic Phospholipase A2 (phospho S505) 抗体 (ab53105)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2 (phospho S505)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytosolic Phospholipase A2 (phospho S505) antibody
Cytosolic Phospholipase A2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytosolic Phospholipase A2 (phospho S505) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Cytosolic Phospholipase A2 (phospho S505).
Sequence:P-L-Sp-P-L
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab53105 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53105の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/300 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 85 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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WB
1/300 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 85 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
組織特異性
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
配列類似性
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
ドメイン
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
翻訳後修飾
Activated by phosphorylation at both Ser-505 and Ser-727. -
細胞内局在
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
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参照データベース
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Omim: 600522 Human
- SwissProt: P47712 Human
- SwissProt: Q6P1J6 Human
- Unigene: 444933 Human
- Unigene: 497200 Human
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別名
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
画像
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Western blot - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105)All lanes : Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105) at 1/300 dilution
Lane 1 : extracts from HeLa cells treated
with TNF-a (20ng/ml, 30mins)
Lane 2 : extracts from HeLa cells treated
with TNF-a (20ng/ml, 30mins) with immunising peptide
Predicted band size: 85 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105)ab53105 at 1/50 dilution staining Cytosolic Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Cytosolic Phospholipase A2 (phospho S505) antibody (ab53105)ICC/IF image of ab53105 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53105, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab53105 は 15 報の論文で使用されています。
- Sun J et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharm Biol 59:672-682 (2021). PubMed: 34078224
- Xue Y et al. Gigantol ameliorates CCl4-induced liver injury via preventing activation of JNK/cPLA2/12-LOX inflammatory pathway. Sci Rep 10:22265 (2020). PubMed: 33335297
- Tunset HM et al. Cytosolic Phospholipase A2 Alpha Regulates TLR Signaling and Migration in Metastatic 4T1 Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31569627
- González-Rodríguez P et al. Brain-derived neurotrophic factor alleviates the oxidative stress induced by oxygen and glucose deprivation in an ex vivo brain slice model. J Cell Physiol 234:9592-9604 (2019). PubMed: 30317637
- Hartz AMS et al. Cytosolic phospholipase A2 is a key regulator of blood-brain barrier function in epilepsy. FASEB J 33:14281-14295 (2019). PubMed: 31661303