AB109452

Anti-Cytokeratin 8 (phospho S431) 抗体 [EP1630]

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 3 publications.

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Cytokeratin 8 with purified ab109452 at 1/500 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.26 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

ab109452 at 1/100 dilution staining Cytokeratin 8 in Human ductal breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

ab109452 at 1/100 dilution staining Cytokeratin 8 in Human stomach adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Purified ab109452 at 1/20 dilution (0.5μg) immunoprecipitating Cytokeratin 8 in HeLa treated with Calyculin A whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A whole cell lysate 10μg
Lane 2 (+) : ab109452 + HeLa treated with Calyculin A whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109452 in HeLa treated with Calyculin A whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 52 kDa

All lanes:

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452)

Predicted band size: 53 kDa

false

Unknown

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

All lanes:

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (ab109452) at 1/10000 dilution

Lane 1:

HeLa lysates, untreated at 10 µg

Lane 2:

HeLa lysates, treated with FBS and Calyculin A at 10 µg

Predicted band size: 53 kDa

Observed band size: 52 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1630

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 79:95 PubMed35080691

2022

Autophagosome-lysosome fusion is facilitated by plectin-stabilized actin and keratin 8 during macroautophagic process.

Applications

Unspecified application

Species

Unspecified reactive species

Sumin Son,Ahruem Baek,Jong Hun Lee,Dong-Eun Kim

Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing 26:297-308 PubMed33691026

2021

A multi-scale integrated analysis identifies KRT8 as a pan-cancer early biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Madeleine K D Scott,Maneesha Limaye,Steven Schaffert,Robert West,Michael G Ozawa,Pauline Chu,Viswam S Nair,Albert C Koong,Purvesh Khatri

Journal of cell science 126:4721-31 PubMed23902688

2013

A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer.

Applications

Unspecified application

Species

Saccharomyces cerevisiae S288C

Takahisa Kuga,Hideaki Kume,Naoko Kawasaki,Misako Sato,Jun Adachi,Takashi Shiromizu,Isamu Hoshino,Takanori Nishimori,Hisahiro Matsubara,Takeshi Tomonaga

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 8 (phospho S431) 抗体 [EP1630]

Anti-Cytokeratin 8 (phospho S431) antibody [EP1630]

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Immunoprecipitation - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Western blot - Anti-Cytokeratin 8 (phospho S431) antibody [EP1630] (AB109452)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (3)

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Abcam product promise