Anti-Cytokeratin 8 (phospho S23) 抗体 [EP1629Y] (ab76584)
![Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)](https://www.abcam.co.jp/ps/products/76/ab76584/Images/ab76584-535037-anti-cytokeratin-8-phospho-s23-antibody-ep1629y-western-blot-human-hela.jpg "Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1629Y] to Cytokeratin 8 (phospho S23)
- Suitable for: Dot blot, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y]
Cytokeratin 8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1629Y] to Cytokeratin 8 (phospho S23) -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: HeLa lysates. IHC-P: Human stomach tissue. Dot Blot: Cytokeratin 8 (phospho S23) peptide.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1629Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76584の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB |
1/10000. Predicted molecular weight: 54 kDa.
|
|
| IHC-P |
1/3600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
Dot blot
1/1000. |
|
WB
1/10000. Predicted molecular weight: 54 kDa. |
|
IHC-P
1/3600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
組織特異性
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
関連疾患
Cirrhosis -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
細胞内局在
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
-
参照データベース
- Entrez Gene: 3856 Human
- Omim: 148060 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
-
別名
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
see all
画像
-
Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)All lanes : Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/10000 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with 100nM Calyculin A for 30mins whole cell lysate
Lane 3 : HeLa treated with 100nM Calyculin A for 30mins whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 7 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)](https://www.abcam.co.jp/ps/products/76/ab76584/Images/ab76584-535038-anti-cytokeratin-8-phospho-s23-antibody-ep1629y-immunohistochemistry-human-stomach.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Cytokeratin 8 with ab76584 at 1/3600 dilution (0.035 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab76584 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)](https://www.abcam.co.jp/ps/products/76/ab76584/Images/ab76584-535039-anti-cytokeratin-8-phospho-s23-antibody-ep1629y-dot-blot-human.jpg)
Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584)Dot blot analysis of Cytokeratin 8 (phospho S23) using ab76584 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100,000 dilution. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 180s.
Lane 1: Cytokeratin 8 (phospho S23) peptide
Lane 2: EGFR non-phospho peptide
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab76584 は 2 報の論文で使用されています。
- Kuga T et al. FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes. Sci Rep 6:26557 (2016). PubMed: 27222304
- Kakehashi A et al. Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas. Toxicol Appl Pharmacol 238:71-9 (2009). IHC-Fr . PubMed: 19409407