AB76584

Anti-Cytokeratin 8 (phospho S23) 抗体 [EP1629Y]

Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y]

BOND RX™ Validated
RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 8 phospho S23 antibody. Suitable for IHC-P, WB, Dot and reacts with Human, Synthetic peptide samples. Cited in 3 publications.

別名を表示する

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Cytokeratin 8 with ab76584 at 1/3600 dilution (0.035 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab76584 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 epitope retrieval solution 2) for 20 mins.

Supplier Data

Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/10000 dilution

Lane 1:

Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HeLa treated with 100nM Calyculin A for 30mins whole cell lysate at 15 µg

Lane 3:

HeLa treated with 100nM Calyculin A for 30mins whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Exposure time: 7s

Supplier Data

Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Dot blot analysis of Cytokeratin 8 (phospho S23) using ab76584 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100,000 dilution. Blocking and dilution buffer : 5% NFDM/TBST. Exposure time : 180s.

All lanes:

Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/1000 dilution

Lane 1:

Cytokeratin 8 (phospho S23) peptide

Lane 2:

EGFR non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1629Y

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

Dot, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/3600", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "" } } }

style

left-column

製品の詳細

This product has switched from a hybridoma to recombinant production method on 23rd February 2024.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8 phospho S23

style

left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 59:2347-2363.e9 PubMed38843837

2024

An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration.

Applications

Unspecified application

Species

Unspecified reactive species

Shifaan Thowfeequ,Jonathan Fiorentino,Di Hu,Maria Solovey,Sharon Ruane,Maria Whitehead,Felix Zhou,Jonathan Godwin,Yentel Mateo-Otero,Bart Vanhaesebroeck,Antonio Scialdone,Shankar Srinivas

Scientific reports 6:26557 PubMed27222304

2016

FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Takahisa Kuga,Mitsuho Sasaki,Toshinari Mikami,Yasuo Miake,Jun Adachi,Maiko Shimizu,Youhei Saito,Minako Koura,Yasunori Takeda,Junichiro Matsuda,Takeshi Tomonaga,Yuji Nakayama

Toxicology and applied pharmacology 238:71-9 PubMed19409407

2009

Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas.

Applications

IHC-Fr

Species

Unspecified reactive species

Anna Kakehashi,Masayo Inoue,Min Wei,Shoji Fukushima,Hideki Wanibuchi

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 8 (phospho S23) 抗体 [EP1629Y]

Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise