Anti-Cytokeratin 7+17 抗体 [C-46] (ab670)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/ab670-3606-anti-cytokeratin-717-antibody-c-46-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)")
Key features and details
- Mouse monoclonal [C-46] to Cytokeratin 7+17
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-Cytokeratin 7+17 antibody [C-46] -
製品の詳細
Mouse monoclonal [C-46] to Cytokeratin 7+17 -
由来種
Mouse -
特異性
Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa) -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat, Rabbit -
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 7+17. Cytoskeleton preparation of HeLa cells (cervix carcinoma)
-
ポジティブ･コントロール
- IHC-P: Human Pancreas tissue WB: HeLa
-
特記事項
Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
C-46 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab670の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml.
Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hot Laemmli buffer (reducing conditions) |
|
| IHC-P |
Use a concentration of 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication, 5 min. in hot Laemmli buffer (reducing conditions) |
|
IHC-P
Use a concentration of 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB |
ターゲット情報
-
関連性
Cytokeratin 7 and 17 is a heterotetramer of a type I and type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Cytokeratin 17 is required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3855 Human
- Entrez Gene: 3872 Human
- SwissProt: P08729 Human
- SwissProt: Q04695 Human
-
別名
- CK17 antibody
- CK7 antibody
- Cytokeratin 17 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.
-
![Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/ab670-473353-anticytokeratin-717-antibody-c46-western-blot-hela.jpg)
Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)All lanes : Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 2 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Non-Reducing)
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (Non-Reducing)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (Reducing)
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (Reducing)
Secondary
All lanes : IRDye800-conjugated anti-mouse IgG1 secondary antibody
Observed band size: 46,54 kDa why is the actual band size different from the predicted?A specific band was detected for cytokeratin 17 at approximately 46 kDa and for cytokeratin 7 at approximately 54 kDa.
-
![Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.co.jp/ps/products/0/ab670/Images/ab670-3607-anti-cytokeratin-717-antibody-c-46-western-blot.jpg)
Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate
Observed band size: 49,51 kDa why is the actual band size different from the predicted?
Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab670 は 3 報の論文で使用されています。
- Hegstad J et al. Female rat sexual behavior is unaffected by perinatal fluoxetine exposure. Psychoneuroendocrinology 120:104796 (2020). PubMed: 32711369
- Sun Y et al. Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis. Toxicology 262:162-70 (2009). PubMed: 19524636
- Vojtesek B et al. A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma 37:333-42 (1990). PubMed: 1695327