Anti-Cytokeratin 7 抗体 [RCK105] - Cytoskeleton Marker (ab9021)

Thank you for contacting us.

You had previously been in contact with my colleague heather regarding staining observed in your negative control using these mouse monoclonal Cytokeratin antibodies on mouse tissue. To clarify, is your negative control from a tissue that is not expected to express cytokeratin or is it an isotype control or a no primary antibody control?

When using an anti-mouse secondary antibody on mouse tissue, background staining is often observed due to cross-reactivity with endogenous mouse IgGs in the sample. This can be checked by testing a no-primary antibody control slide.

If you are observing mouse on mouse background staining, this can be avoided by either using a mouse on mouse (MOM) staining kit, such as ab127055. The background can also be blocked by adding an incubation with a F(ab) fragment unconjugated anti-mouse secondary antibody, such as ab6668. The F(ab) fragment will coat any endogenous IgGs in your sample, and can be done just before the serum block at a 1:500 dilution for 1 hour at room temperature.

I hope this helps, if not, please let me know and I will be happy to help you further.

I apologize for the delay.

Vimentin is a mesenchymal cell marker, ab8978 is tested in Zebrafish. It might be suitable for your purpose.

ab9004, ab9021, ab9217 are anti Cytokeratin 4,7 and 18. Against epithelial cell marker the antibodies is tested in Zebrafish.

Could you more specific to type of blood cell marker you are interested in?

We do not have anti M-Cadherin and Myo-D antibodies that could be used for Zebrafish. This assumption is based on the protein sequence similarity between human and Zebrafish. We unfortunately do not have antibodies against these target that might cross react.

I have attached a Excel file of all Zebrafish specific antibodies with immunogen sequence. This file will help you selecting the antibodies based on cross reactivity due to immunogen sequence homology.

I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


Abpromise guarantee:

We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab52870.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.