Anti-Cytokeratin 7 抗体 (ab53123)
Key features and details
- Rabbit polyclonal to Cytokeratin 7
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytokeratin 7 antibody
Cytokeratin 7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytokeratin 7 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
A synthetic peptide derived from human Cytokeratin 7.
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ポジティブ･コントロール
- HepG2 cell extracts and human breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab53123 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53123の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| WB |
1/300 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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WB
1/300 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
組織特異性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Arg-20 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3855 Human
- Omim: 148059 Human
- SwissProt: P08729 Human
- Unigene: 411501 Human
- Unigene: 670221 Human
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別名
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (ab53123)ab53123 at 1/50 dilution staining Cytokeratin 7 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
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Western blot - Anti-Cytokeratin 7 antibody (ab53123)All lanes : Anti-Cytokeratin 7 antibody (ab53123) at 1/300 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide
Predicted band size: 51 kDa
Observed band size: 51 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (ab53123)ICC/IF image of ab53123 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab53123 は 6 報の論文で使用されています。
- Afshar Y et al. Circulating trophoblast cell clusters for early detection of placenta accreta spectrum disorders. Nat Commun 12:4408 (2021). PubMed: 34344888
- Johnson LJ et al. Human Placental Trophoblasts Infected by Listeria monocytogenes Undergo a Pro-Inflammatory Switch Associated With Poor Pregnancy Outcomes. Front Immunol 12:709466 (2021). PubMed: 34367171
- Azari S et al. Hofbauer Cells Spread Listeria monocytogenes among Placental Cells and Undergo Pro-Inflammatory Reprogramming while Retaining Production of Tolerogenic Factors. mBio 12:e0184921 (2021). PubMed: 34399615
- Ehrenberg KR et al. Systematic Generation of Patient-Derived Tumor Models in Pancreatic Cancer. Cells 8:N/A (2019). PubMed: 30744205
- Rakers S et al. Monitoring changing cellular characteristics during the development of a fin cell line from Cyprinus carpio. Comp Biochem Physiol B Biochem Mol Biol 225:1-12 (2018). PubMed: 29960082
- Ball CR et al. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts. EMBO Mol Med 9:918-932 (2017). IHC-P, ICC/IF . PubMed: 28526679