Anti-Cytokeratin 7 抗体 (ab154334)
Key features and details
- Rabbit polyclonal to Cytokeratin 7
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cytokeratin 7 antibody
Cytokeratin 7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytokeratin 7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IHC-Fr, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Monkey
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免疫原
Recombinant fragment, corresponding to a region within amino acids 44-319 of Human Cytokeratin 7 (Uniprot ID: P08729).
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ポジティブ･コントロール
- A431, HeLa and MOLT4 whole cell lysates; HeLa cells; Human placenta tissue and A549 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154334の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/5000 - 1/20000. Predicted molecular weight: 51 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IHC-Fr |
1/100 - 1/1000.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/5000 - 1/20000. Predicted molecular weight: 51 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IHC-Fr
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). -
組織特異性
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Arg-20 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 535697 Cow
- Entrez Gene: 3855 Human
- Entrez Gene: 110310 Mouse
- Entrez Gene: 300242 Rat
- Omim: 148059 Human
- SwissProt: Q29S21 Cow
- SwissProt: P08729 Human
- SwissProt: Q9DCV7 Mouse
see all -
別名
- CK 7 antibody
- CK-7 antibody
- CK7 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody (ab154334)Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling Cytokeratin 7 with ab154334 at 1/500 dilution.
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Western blot - Anti-Cytokeratin 7 antibody (ab154334)Anti-Cytokeratin 7 antibody (ab154334) at 1/10000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 51 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 7 antibody (ab154334)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Cytokeratin 7 with ab154334 at 1/200 dilution. Lower image is merged with DNA probe. -
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody (ab154334)Immunohistochemical analysis of Human placenta tissue (paraformaldehyde-fixed frozen sections), labeling Cytokeratin 7 with ab154334 at 1/200 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154334 は 3 報の論文で使用されています。
- Su Y et al. Exosomes derived from placental trophoblast cells regulate endometrial epithelial receptivity in dairy cows during pregnancy. J Reprod Dev 68:21-29 (2022). PubMed: 34690214
- Chen J et al. Patient-Derived Xenografts Are a Reliable Preclinical Model for the Personalized Treatment of Epithelial Ovarian Cancer. Front Oncol 11:744256 (2021). PubMed: 34671560
- Wang Y & Li H Identification of proteins associated with paclitaxel resistance of epithelial ovarian cancer using iTRAQ-based proteomics. Oncol Lett 15:9793-9801 (2018). PubMed: 29928353