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AB18586

Anti-Cytokeratin 6 抗体 [Ks6.KA12]

Anti-Cytokeratin 6 antibody [Ks6.KA12]

5

(4 Reviews)

|

(17 Publications)

Mouse Monoclonal K2C6C antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT6C.

別名を表示する

KRT6E, KRT6C, Cytokeratin-6C, Cytokeratin-6E, Keratin K6h, Keratin-6C, Type-II keratin Kb12, CK-6C, CK-6E, K6C

1 Images
Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (AB18586)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 6 antibody [Ks6.KA12] (AB18586)

Overlay histogram showing A431 cells stained with ab18586 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18586, 2μg/1x106 cells ) for 30 min at 22°C. The secondary antibody used was a goat anti-mous DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1]ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

Ks6.KA12

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Recombinant Full Length Protein corresponding to Human KRT6C.

P48668

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Purified by protein A affinity chromatography.
バッファー組成
pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 0.5% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

See full target information KRT6C

文献 (17)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 65:8 PubMed38315493

2024

Urban Particulate Matter Triggers Meibomian Gland Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqian Tu,Ren Liu,Jianwen Xue,Bing Xiao,Jing Li,Lingyi Liang

Skin health and disease 3:e245 PubMed37799359

2023

Inhibition of vascular endothelial growth factor-A downregulates angiogenesis in psoriasis: A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Luengas-Martinez,Dina Ismail,Ralf Paus,Helen S Young

International journal of molecular sciences 24: PubMed37047109

2023

Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Song Cui,So Young Joo,Seung Yeol Lee,Yoon Soo Cho,Dong Hyun Kim,Cheong Hoon Seo

Biomedicines 11: PubMed36672635

2023

New Data on the Features of Skin Barrier in Hidradenitis Suppurativa.

Applications

Unspecified application

Species

Unspecified reactive species

Orsolya Somogyi,Zsolt Dajnoki,Lilla Szabó,Krisztián Gáspár,Zoltán Hendrik,Christos C Zouboulis,Klaudia Dócs,Péter Szücs,Katalin Dull,Dániel Törőcsik,Anikó Kapitány,Andrea Szegedi

Frontiers in medicine 9:981610 PubMed36148459

2022

Expression of extracellular matrix components in the meibomian gland.

Applications

Unspecified application

Species

Unspecified reactive species

Di Chen,Xiaomin Chen,Hua-Tao Xie,Mark P Hatton,Xiaowei Liu,Yang Liu

iScience 25:104983 PubMed36093061

2022

Heme oxygenase-1 mitigates liver injury and fibrosis via modulation of LNX1/Notch1 pathway in myeloid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giacomo Canesin,Linda Feldbrügge,Guangyan Wei,Lubica Janovicova,Monika Janikova,Eva Csizmadia,Juliana Ariffin,Andreas Hedblom,Zachary T Herbert,Simon C Robson,Peter Celec,Kenneth D Swanson,Imad Nasser,Yury V Popov,Barbara Wegiel

Scientific reports 11:17296 PubMed34453089

2021

Effect of extracorporeal shock wave therapy on keratinocytes derived from human hypertrophic scars.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Song Cui,So Young Joo,Yoon Soo Cho,Ji Heon Park,Yu Mi Ro,June-Bum Kim,Cheong Hoon Seo

Biomolecules 11: PubMed34203873

2021

Comprehensive Evaluation of PAXgene Fixation on Oral Cancer Tissues Using Routine Histology, Immunohistochemistry, and FTIR Microspectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Pooja Lahiri,Suranjana Mukherjee,Biswajoy Ghosh,Debnath Das,Basudev Lahiri,Shailendra Kumar Varshney,Mousumi Pal,Ranjan Rashmi Paul,Jyotirmoy Chatterjee

International wound journal 18:332-341 PubMed33751815

2021

Epidermal graft encourages wound healing by down-regulation of gap junctional protein and activation of wound bed without graft integration as opposed to split-thickness skin graft.

Applications

Unspecified application

Species

Unspecified reactive species

Muholan Kanapathy,Nadine Hachach-Haram,Nicola Bystrzonowski,David L Becker,Afshin Mosahebi,Toby Richards

Thyroid : official journal of the American Thyroid 30:1066-1078 PubMed32111151

2020

The Thyroid Hormone Inactivator Enzyme, Type 3 Deiodinase, Is Essential for Coordination of Keratinocyte Growth and Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppina Mancino,Annarita Sibilio,Cristina Luongo,Emery Di Cicco,Caterina Miro,Annunziata Gaetana Cicatiello,Annarita Nappi,Serena Sagliocchi,Raffaele Ambrosio,Maria Angela De Stefano,Daniela Di Girolamo,Tommaso Porcelli,Melania Murolo,Federica Saracino,Giuseppe Perruolo,Pietro Formisano,Mariano Stornaiuolo,Monica Dentice
View all publications

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