Anti-Cytokeratin 5 抗体 [SP178] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Cytokeratin 5 with ab183336 at 1/100 dilution (2.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183336)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 5 with purified ab183336 at 1/50 (4.7 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183336).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 5 with purified ab183336 at 1/20 dilution (11.75μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183336).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human rectal carcinoma tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling Cytokeratin 5 with ab183336 at 1/100 dilution (2.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183336)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung squamous cell carcinoma tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Cytokeratin 5 with ab183336 at 1/100 dilution (2.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183336)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin squamous cell carcinoma tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervix squamous cell carcinoma tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free (AB243922)

Intracellular flow cytometric analysis of A431 cells labeling Cytokeratin 5 with ab183336 at 1/100 dilution (green) compared to a negative control rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183336).