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AB183336

Anti-Cytokeratin 5 抗体 [SP178]

Anti-Cytokeratin 5 antibody [SP178]

0

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal Cytokeratin 5 antibody. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

別名を表示する

58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5, CK5

15 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Cytokeratin 5 with ab183336 at 1/100 dilution (2.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 5 with purified ab183336 at 1/50 (4.7 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Flow Cytometry analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 5 with purified ab183336 at 1/20 dilution (11.75 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human rectal carcinoma tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling Cytokeratin 5 with ab183336 at 1/100 dilution (2.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung squamous cell carcinoma tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Cytokeratin 5 with ab183336 at 1/100 dilution (2.35 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin squamous cell carcinoma tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervix squamous cell carcinoma tissue labeling Cytokeratin 5 with ab183336 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [SP178] (AB183336)

Flow cytometric analysis of A431 cells labeling Cytokeratin 5 with ab183336 at 1/100 dilution (green) compared to a negative control rabbit IgG (blue).

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Cytokeratin 5 antibody [SP178] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP178

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, Flow Cyt (Intra), ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB224985

PE Anti-Cytokeratin 5 antibody [EP1601Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 5 antibody [EP1601Y] (AB224985)

  • 0
  • 0
Primary Antibodies

AB224984

APC Anti-Cytokeratin 5 antibody [EP1601Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - APC Anti-Cytokeratin 5 antibody [EP1601Y] (AB224984)

  • 0
  • 0
Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

  • 5
  • 11
Primary Antibodies

AB193895

Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y] (AB193895)

  • 5
  • 4
Primary Antibodies

AB214586

Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free (AB214586)

  • 5
  • 2
Primary Antibodies

AB75869

Anti-Cytokeratin 5 antibody [EPR1600Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Cytokeratin 5 antibody [EPR1600Y] (AB75869)

  • 4
  • 1
Primary Antibodies

AB193894

Alexa Fluor® 488 Anti-Cytokeratin 5 antibody [EP1601Y]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 5 antibody [EP1601Y] (AB193894)

  • 0
  • 0

組合せ製品

Assay Kits

AB226896

Human Cytokeratin 5 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Cytokeratin 5 ELISA Kit (AB226896)

  • 0
  • 0
Primary Antibodies

AB52455

Anti-HLA G antibody [4H84]

Flow Cytometry - Anti-HLA G antibody [4H84] (AB52455)

  • 4
  • 6
Primary Antibodies

AB53280

Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (AB53280)

  • 5
  • 9
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB119695

Anti-Cytokeratin 14 antibody [SP53]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [SP53] (AB119695)

  • 5
  • 6
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/100", "FlowCytIntra-species-notes": "<p>Incubate for 30 minutes at 4°C.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. <br>Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. <br>Primary Antibody Incubation: Incubate for 10 minutes at room temperature. <br>Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from TCS by protein A/G.
バッファー組成
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).
See full target information KRT5
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0125255 PubMed25955027

2015

Initiation and characterization of small cell lung cancer patient-derived xenografts from ultrasound-guided transbronchial needle aspirates.

Applications

Unspecified application

Species

Unspecified reactive species

Wade C Anderson,Michael B Boyd,Jorge Aguilar,Brett Pickell,Amy Laysang,Marybeth A Pysz,Sheila Bheddah,Johanna Ramoth,Brian C Slingerland,Scott J Dylla,Edmundo R Rubio
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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