Anti-Cytokeratin 3/CK-3 抗体 [AE5] (ab68260)
![Western blot - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)](https://www.abcam.co.jp/ps/products/68/ab68260/Images/ab68260-506560-anti-cytokeratin-3-ck-3-antibody-ae5-western-blot-transfected-293t-human.jpg "Western blot - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AE5] to Cytokeratin 3/CK-3
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Cytokeratin 3/CK-3 antibody [AE5]
Cytokeratin 3/CK-3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AE5] to Cytokeratin 3/CK-3 -
由来種
Mouse -
特異性
This antibody recognizes the 64kD polypeptide (cytokeratin 3 / CK-3) and 55 kDa polypeptide (cytokeratin 12) of rabbit corneal epithelium. It also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line. The clone number has been updated from (2Q1040) to (AE5) both clone numbers name the same antibody clone.
-
アプリケーション
適用あり: IHC-Pmore details
適用なし: Flow Cyt (Intra),ICC/IF,IP or WB -
種交差性
交差種: Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- IHC: Human squamous papilloma of cornea tissue
-
特記事項
This product has switched from a hybridoma to recombinant production method on 5th January 2022
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
AE5 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68260の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 0.25 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 0.25 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
-
組織特異性
Cornea specific. -
関連疾患
Defects in KRT3 are a cause of Meesmann corneal dystrophy (MECD) [MIM:122100]; also abbreviated as MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. -
配列類似性
Belongs to the intermediate filament family. - Information by UniProt
-
参照データベース
- Entrez Gene: 3850 Human
- Omim: 148043 Human
- SwissProt: P12035 Human
- Unigene: 680652 Human
-
別名
- 65 kDa cytokeratin antibody
- CK-3 antibody
- CK3 antibody
see all
画像
-
Western blot - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)All lanes : Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260) at 1/5000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector containing a His tag®, whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with Cytokeratin full length expression vector containing a His tag®, whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 64 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)](https://www.abcam.co.jp/ps/products/68/ab68260/Images/ab68260-482926-anti-cytokeratin-3-antibody-immunohistochemistry-squamous-papilloma-of-cornea-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 3/CK-3 antibody [AE5] (ab68260)Immunohistochemical analysis of paraffin-embedded human squamous papilloma of cornea tissue labelling Cytokeratin 3 with ab68260 at 1/500 dilution, followed by a ready-to-use secondary antibody. Cytosolic staining on human squamous papilloma of cornea tissue is observed. Counterstained with Hematoxylin. Positive staining on human squamous papilloma of cornea. The section was incubated with ab68260 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (34)
ab68260 は 34 報の論文で使用されています。
- Logan CM et al. In Situ-forming Collagen Hydrogels Crosslinked by Multifunctional Polyethylene Glycol as a Matrix Therapy for Corneal Defects: 2-Month Follow-up In Vivo. Cornea 42:97-104 (2023). PubMed: 35965399
- Yu Z et al. Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process. STAR Protoc 4:102122 (2023). PubMed: 36861830
- Zekušić M et al. New characterization and safety evaluation of human limbal stem cells used in clinical application: fidelity of mitotic process and mitotic spindle morphologies. Stem Cell Res Ther 14:368 (2023). PubMed: 38093301
- Jiang T et al. Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide. Tissue Eng Regen Med 19:403-415 (2022). PubMed: 35122584
- Jaffet J et al. Clinical, histological and immunohistochemistry characteristics of cornea in the sequelae stage of chronic vernal keratoconjunctivitis. Indian J Ophthalmol 70:59-64 (2022). PubMed: 34937208