Anti-Cytokeratin 19 抗体 (ab53119)
Key features and details
- Rabbit polyclonal to Cytokeratin 19
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytokeratin 19 antibody
Cytokeratin 19 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytokeratin 19 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide derived form human Cytokeratin 19.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53119の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| WB | (1) |
1/300 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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WB
1/300 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
組織特異性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
Present in hair follicles at all stages of development. -
ドメイン
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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参照データベース
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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別名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody (ab53119)ab53119 at 1/50 dilution staining Cytokeratin 19 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
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Western blot - Anti-Cytokeratin 19 antibody (ab53119)All lanes : Anti-Cytokeratin 19 antibody (ab53119) at 1/300 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract with immunising peptide
Predicted band size: 44 kDa
Observed band size: 44 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody (ab53119)ICC/IF image of ab53119 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53119, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab53119 は 10 報の論文で使用されています。
- Sitia L et al. Selective Targeting of Cancer-Associated Fibroblasts by Engineered H-Ferritin Nanocages Loaded with Navitoclax. Cells 10:N/A (2021). PubMed: 33562504
- Carpino G et al. Neoplastic Transformation of the Peribiliary Stem Cell Niche in Cholangiocarcinoma Arisen in Primary Sclerosing Cholangitis. Hepatology 69:622-638 (2019). PubMed: 30102768
- Razumilava N et al. Hedgehog Signaling Modulates Interleukin-33-Dependent Extrahepatic Bile Duct Cell Proliferation in Mice. Hepatol Commun 3:277-292 (2019). PubMed: 30766964
- Kim EM et al. Connexin 43 plays an important role in the transformation of cholangiocytes with Clonochis sinensis excretory-secretory protein and N-nitrosodimethylamine. PLoS Negl Trop Dis 13:e0006843 (2019). PubMed: 30943209
- Quaranta V et al. Macrophage-Derived Granulin Drives Resistance to Immune Checkpoint Inhibition in Metastatic Pancreatic Cancer. Cancer Res 78:4253-4269 (2018). PubMed: 29789416
- Yang S et al. Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype. Int J Mol Med 37:1263-73 (2016). IHC-P, WB ; Human . PubMed: 26986690
- Miura N et al. Efficacy of adjuvant chemotherapy for non-small cell lung cancer assessed by metastatic potential associated with ACTN4. Oncotarget 7:33165-78 (2016). PubMed: 27121206
- Eidet JR et al. The impact of storage temperature on the morphology, viability, cell number and metabolism of cultured human conjunctival epithelium. Curr Eye Res 40:30-9 (2015). PubMed: 24750037
- Zhao Z et al. Direct reprogramming of human fibroblasts into sweat gland-like cells. Cell Cycle 14:3498-505 (2015). PubMed: 26566868
- Morris EV et al. Nuclear iASPP may facilitate prostate cancer progression. Cell Death Dis 5:e1492 (2014). PubMed: 25341046