Anti-Cytokeratin 19 抗体 (ab15463)
Key features and details
- Rabbit polyclonal to Cytokeratin 19
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Cytokeratin 19 antibody
Cytokeratin 19 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytokeratin 19 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human Cytokeratin 19 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: P08727 -
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15463の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
|
|
IHC-P | (9) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil in citrate buffer for 10 minutes, cool at room temperature for 20 minutes. After blocking, incubate primary antibody 1:100 for 10 minutes at room temperature. |
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil in citrate buffer for 10 minutes, cool at room temperature for 20 minutes. After blocking, incubate primary antibody 1:100 for 10 minutes at room temperature. |
ターゲット情報
-
機能
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
組織特異性
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
Present in hair follicles at all stages of development. -
ドメイン
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
-
参照データベース
- Entrez Gene: 3880 Human
- Entrez Gene: 16669 Mouse
- Omim: 148020 Human
- SwissProt: P08727 Human
- SwissProt: P19001 Mouse
- Unigene: 654568 Human
- Unigene: 439699 Mouse
-
別名
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
画像
-
Human skin stained with anti-Cytokeratin 19 antibody (ab15463).
-
All lanes : Anti-Cytokeratin 19 antibody (ab15463) at 1 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. -
ab15463 staining Cytokeratin 19 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 22°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse developing skin tissue labeling Cytokeratin 19 with ab15463 at 1/500 dilution. The tissue was fixed with formaldehyde; heat-mediated antigen retrieval was performed using a citric acid. Tissue was blocked with 2% BSA for 10 minutes at 21°C. A polyclonal goat anti-rabbit IgG biotin conjugated antibody was used ar 1/300 dilution. Image shows strong immunopositivity in the cytoplasm of basal cells of the epidermis and a moderate hair follicle cell positivity.
-
This image shows formalin-fixed mouse skin stained with Cytokeratin 19 antibody (1/50 dilution) after pepsin digestion.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (79)
ab15463 は 79 報の論文で使用されています。
- Obata T et al. MicroRNA-451a inhibits gemcitabine-refractory biliary tract cancer progression by suppressing the MIF-mediated PI3K/AKT pathway. Mol Ther Nucleic Acids 34:102054 (2023). PubMed: 38111913
- Shirakami Y et al. A Novel Mouse Model of Intrahepatic Cholangiocarcinoma Induced by Azoxymethane. Int J Mol Sci 24:N/A (2023). PubMed: 37834032
- Dias ML et al. Cirrhotic Liver Sustains In Situ Regeneration of Acellular Liver Scaffolds after Transplantation into G-CSF-Treated Animals. Cells 12:N/A (2023). PubMed: 37048049
- Mohan S et al. Ac3IV, a V1a and V1b receptor selective vasopressin analogue, protects against hydrocortisone-induced changes in pancreatic islet cell lineage. Peptides 152:170772 (2022). PubMed: 35202749
- Polvani S et al. Isoforms of the orphan nuclear receptor COUP‑TFII differentially modulate pancreatic cancer progression. Int J Oncol 60:N/A (2022). PubMed: 35348189