JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB212566

Anti-Cytokeratin 19 抗体 [A53-B/A2.26] - BSA and Azide free

Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal Cytokeratin 19 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing KRT19 protein.

別名を表示する

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (AB212566)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (AB212566)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 19 using ab212566 at 1 μg/ml in immunohistochemical analysis.

This IHC-P data was generated using the same anti-Cytokeratin 19 antibody clone, A53-B/A2.26, in a different buffer formulation.

Western blot - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (AB212566)
  • WB

Supplier Data

Western blot - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (AB212566)

This IHC-P data was generated using the same anti-Cytokeratin 19 antibody clone, A53-B/A2.26, in a different buffer formulation.

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (ab212566)

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 44 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

A53-B/A2.26

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

Cell preparation containing KRT19 protein. The exact immunogen used to generate this antibody is proprietary information.

P08727

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Ferret": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
ab212566 was purified from Bioreactor Concentrate by Protein A/G.
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokera­tine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.
Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
See full target information KRT19

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:4906 PubMed34385456

2021

An N-Cadherin 2 expressing epithelial cell subpopulation predicts response to surgery, chemotherapy and immunotherapy in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth H Gouin,Nathan Ing,Jasmine T Plummer,Charles J Rosser,Bassem Ben Cheikh,Catherine Oh,Stephanie S Chen,Keith Syson Chan,Hideki Furuya,Warren G Tourtellotte,Simon R V Knott,Dan Theodorescu
View all publications

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com