Anti-Cytokeratin 18 (phospho S33) 抗体 (ab75747)
Key features and details
- Rabbit polyclonal to Cytokeratin 18 (phospho S33)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytokeratin 18 (phospho S33) antibody
Cytokeratin 18 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytokeratin 18 (phospho S33) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Cytokeratin 18 (phospho S33).
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ポジティブ･コントロール
- Human breast carcinoma tissue; extracts from A431 cells and EC-304 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab75747 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75747の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 48 kDa.
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 48 kDa. |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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別名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 (phospho S33) antibody (ab75747)ab75747, at a 1/50 dilution, staining Cytokeratin 18 in paraffin embedded human breast carcinoma tissue by Immunohistochemistrytissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunizing peptide.
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Western blot - Anti-Cytokeratin 18 (phospho S33) antibody (ab75747)All lanes : Anti-Cytokeratin 18 (phospho S33) antibody (ab75747) at 1/500 dilution
Lane 1 : A431 cell extracts with immunising peptide
Lane 2 : A431 cell extracts
Lane 3 : EC-304 cell extracts
Predicted band size: 48 kDa
Observed band size: 48 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab75747 は論文での使用が確認できていません。