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Anti-Cytokeratin 18 抗体 [RGE53] (ab9217)

Reviews (1)Q&A (2)References (8)
Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
  • Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

Key features and details

  • Mouse monoclonal [RGE53] to Cytokeratin 18
  • Suitable for: Flow Cyt, IHC-Fr, WB
  • Reacts with: Human, Pig, Zebrafish
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Cytokeratin 18 antibody [RGE53]
    Cytokeratin 18 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [RGE53] to Cytokeratin 18

  • 由来種

    Mouse

  • 特異性

    This antibody reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them

  • アプリケーション

    適用あり: Flow Cyt, IHC-Fr, WBmore details

  • 種交差性

    交差種: Human, Pig, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Hamster, Dog

  • 免疫原

    Full length protein corresponding to Human Cytokeratin 18. Cytoskeletal preparation of HeLa cells.

  • ポジティブ･コントロール

    • This antibody gave a positive signal in HeLa and MCF7 whole cell lysates.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9217の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
IHC-Fr
1/100 - 1/200.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
特記事項
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
IHC-Fr
1/100 - 1/200.
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

  • 組織特異性

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

  • 関連疾患

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

  • 配列類似性

    Belongs to the intermediate filament family.

  • 翻訳後修飾

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

  • 細胞内局在

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody
    • CK 18 antibody
    • CK-18 antibody
    • CK18 antibody
    • CYK 18 antibody
    • CYK18 antibody
    • Cytokeratin 18 antibody
    • Cytokeratin endo B antibody
    • Cytokeratin-18 antibody
    • K 18 antibody
    • K18 antibody
    • K1C18_HUMAN antibody
    • KA18 antibody
    • Keratin 18 antibody
    • Keratin 18, type I antibody
    • Keratin D antibody
    • keratin, type I cytoskeletal 18 antibody
    • Keratin-18 antibody
    • Krt18 antibody
    see all

画像

  • Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Western blot - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    All lanes : Anti-Cytokeratin 18 antibody [RGE53] (ab9217) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunohistochemistry on frozen section of human kidney epithelium.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9217 reacts with with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.0
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    immunofluorescence staining of epithelial tissues in a 2 days old zebrafish embryo.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)

    Immunofluorescence staining of 1 month old zebrafish embryo.

  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunofluorescence staining images of 9 day old zebrafish embryos.
    ab9217 reacts with intestinal epithelium and epidermis. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry on frozen section of human kidney epithelium
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry on frozen section of human colon
  • Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Immunohistochemistry on frozen section of swine liver hepatocytes
  • Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Flow Cytometry - Anti-Cytokeratin 18 antibody [RGE53] (ab9217)
    Overlay histogram showing MCF7 cells stained with ab9217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9217, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献 (8)

ab9217 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9217 は 8 報の論文で使用されています。

  • Mauro M  et al. Glypican-3 (GPC-3) Structural Analysis and Cargo in Serum Small Extracellular Vesicles of Hepatocellular Carcinoma Patients. Int J Mol Sci 24:N/A (2023). PubMed: 37446098
  • Montalbano M  et al. Modeling of Hepatocytes Proliferation Isolated from Proximal and Distal Zones from Human Hepatocellular Carcinoma Lesion. PLoS One 11:e0153613 (2016). WB, IHC-P ; Human . PubMed: 27074018
  • Montalbano M  et al. Transformation of primary human hepatocytes in hepatocellular carcinoma. Int J Oncol 48:1205-17 (2016). PubMed: 26717856
  • Xu J  et al. Tbx18 is essential for normal development of vasculature network and glomerular mesangium in the mammalian kidney. Dev Biol 391:17-31 (2014). PubMed: 24727670
  • Liu J  et al. Cell-to-cell contact induces human adipose tissue-derived stromal cells to differentiate into urothelium-like cells in vitro. Biochem Biophys Res Commun 390:931-6 (2009). ICC/IF ; Human . PubMed: 19852942
  • Ramaekers F  et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). PubMed: 1690512
  • Puts JJ  et al. Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix. Int J Gynecol Pathol 4:300-13 (1985). PubMed: 2417968
  • Ramaekers F  et al. Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology. Lab Invest 49:353-61 (1983). PubMed: 6193333

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [RGE53]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (RK-13 (Rabbit kidney epithelial cells))
Permeabilization
Yes - Triton-X-100
Specification
RK-13 (Rabbit kidney epithelial cells)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Egi Kardia

Verified customer

投稿 Jun 03 2019

Question

Your Anti-Cytokeratin 18 antibody [RGE53] (ab9217) is listed as working in zebrafish, but is not listed as working with IF. I will be ordering the antibody soon, and will be using it for IF. I can confirm that the antibody works for IF as part of you 100% testing discount if that information would be valuable to you.
Thank you,

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Answer

Thank you for contacting us.

There was error on the datasheet which is being updated. The antibody has been tested for ICC/IF and is fully guaranteed. I am sorry 100% Abreview discount cannot be provided this time. However if you are interested in testing this antibody in Rat or mouse cells the discount code can be provided.

Please let me know if you are interested.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Zebrafish specific antibodies for
- Epithelial cell marker
- Blood cells
- Mesenchymal cells
- Vascular muscle cells
- M-Cadherins
- Myo-D

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Answer

I apologize for the delay.

Vimentin is a mesenchymal cell marker, ab8978 is tested in Zebrafish. It might be suitable for your purpose.

ab9004, ab9021, ab9217 are anti Cytokeratin 4,7 and 18. Against epithelial cell marker the antibodies is tested in Zebrafish.

Could you more specific to type of blood cell marker you are interested in?

We do not have anti M-Cadherin and Myo-D antibodies that could be used for Zebrafish. This assumption is based on the protein sequence similarity between human and Zebrafish. We unfortunately do not have antibodies against these target that might cross react.

I have attached a Excel file of all Zebrafish specific antibodies with immunogen sequence. This file will help you selecting the antibodies based on cross reactivity due to immunogen sequence homology.

I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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