AB31844

Anti-Cytokeratin 18 抗体 [LDK18]

Name: Anti-Cytokeratin 18 抗体 [LDK18] (ab31844) | Abcam
Brand: Abcam Limited
SKU: AB31844
Price: 72000 JPY
Availability: InStock

Anti-Cytokeratin 18 antibody [LDK18]

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(3 Publications)

Mouse Monoclonal Cytokeratin 18 antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human KRT18 aa 400 to C-terminus.

別名を表示する

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [LDK18] (AB31844)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [LDK18] (AB31844)

ICC/IF image of ab31844 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31844 at 5μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [LDK18] (AB31844)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [LDK18] (AB31844)

ab31844 staining human normal colon tissue. Staining is localised to cytoplasm.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Flow Cytometry - Anti-Cytokeratin 18 antibody [LDK18] (AB31844)

Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 18 antibody [LDK18] (AB31844)

Overlay histogram showing MCF7 cells stained with ab31844 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31844, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

LDK18

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

IHC-P, ICC/IF, Flow Cyt

applications

免疫原

Synthetic Peptide within Human KRT18 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05783

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代替製品

Primary Antibodies

AB206091

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [EPR1626]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [EPR1626] (AB206091)

Primary Antibodies

AB133302

Anti-Cytokeratin 18 antibody [EPR1611]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [EPR1611] (AB133302)

Primary Antibodies

AB181597

Anti-Cytokeratin 18 antibody [EPR17347]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

Primary Antibodies

AB133263

Anti-Cytokeratin 18 antibody [EPR1626]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (AB133263)

Primary Antibodies

AB32118

Anti-Cytokeratin 18 antibody [E431-1]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [E431-1] (AB32118)

Primary Antibodies

AB194124

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1] (AB194124)

組合せ製品

Assay Kits

AB227896

Human Cytokeratin 18 ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cytokeratin 18 ELISA Kit (AB227896)

Primary Antibodies

AB53280

Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (AB53280)

Primary Antibodies

AB52625

Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (AB52625)

Primary Antibodies

AB119695

Anti-Cytokeratin 14 antibody [SP53]

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [SP53] (AB119695)

Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 292:19122-19132 PubMed28935668

2017

Chemical reprogramming of mouse embryonic and adult fibroblast into endoderm lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Shangtao Cao,Shengyong Yu,Yan Chen,Xiaoshan Wang,Chunhua Zhou,Yuting Liu,Junqi Kuang,He Liu,Dongwei Li,Jing Ye,Yue Qin,Shilong Chu,Linlin Wu,Lin Guo,Yinxiong Li,Xiaodong Shu,Jiekai Chen,Jing Liu,Duanqing Pei

PLoS biology 11:e1001593 PubMed23824517

2013

A lack of premature termination codon read-through efficacy of PTC124 (Ataluren) in a diverse array of reporter assays.

Applications

Species

Unspecified reactive species

Stuart P McElroy,Toshifumi Nomura,Leah S Torrie,Emma Warbrick,Ulrike Gartner,Gavin Wood,W H Irwin McLean

Journal of proteomics 75:2404-16 PubMed22387131

2012

Keratins in oral cancer: necessity of mass spectrometry for validation of antibody based identifications.

Applications

Species

Human

Amit Fulzele,Siddhi A Malgundkar,Rukmini B Govekar,Anil K D'Cruz,Pankaj Chaturvedi,Asawari Patil,Shubhada V Kane,Surekha M Zingde

View all publications

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