AB669

Anti-Cytokeratin 18 抗体 [DA-7]

Anti-Cytokeratin 18 antibody [DA-7]

Be the first to review this product! Submit a review

(5 Publications)

Mouse Monoclonal Cytokeratin 18 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing KRT18 protein.

別名を表示する

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DA-7] (AB669)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DA-7] (AB669)

ab669 staining Human normal skin. Staining is localized to the cytoplasm.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Flow Cytometry - Anti-Cytokeratin 18 antibody [DA-7] (AB669)

Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 18 antibody [DA-7] (AB669)

Overlay histogram showing MCF7 cells stained with ab669 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab669, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

DA-7

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt, IHC-P

applications

免疫原

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

特異性

Human Cytokeratin peptide 18 (45 kDa).

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/100", "IHCP-species-notes": "<p></p> Perform enzymatic antigen retrieval before commencing with IHC staining protocol." } } }

style

left-column

下記製品にもご興味をお持ちいただけるかもしれません:

AB227896

Human Cytokeratin 18 ELISA Kit

0 Reviews

製品ページを見る

こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

Purity >95% by SDS-PAGE.

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:4593 PubMed30389931

2018

Tissue-resident memory T cells populate the human brain.

Applications

IHC-P

Species

Human

Joost Smolders,Kirstin M Heutinck,Nina L Fransen,Ester B M Remmerswaal,Pleun Hombrink,Ineke J M Ten Berge,René A W van Lier,Inge Huitinga,Jörg Hamann

Journal of cell science 94 ( Pt 3):403-13 PubMed2483723

1989

Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium.

Applications

Unspecified application

Species

Unspecified reactive species

J Taylor-Papadimitriou,M Stampfer,J Bartek,A Lewis,M Boshell,E B Lane,I M Leigh

Folia biologica 35:373-82 PubMed2483834

1989

Monoclonal antibodies recognizing different epitopes of cytokeratin No.18.

Applications

Unspecified application

Species

Unspecified reactive species

B Vojtĕsek,Z Stasková,R Nenutil,L Lauerová,J Kovarík,A Rejthar,J Bártková,J Bártek

Hybridoma 7:495-504 PubMed2461901

1988

Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule.

Applications

Unspecified application

Species

Unspecified reactive species

L Lauerová,J Kovarik,J Bártek,A Rejthar,B Vojtĕsek

International journal of cancer. Supplement = Journal international du cancer. Supplement 3:50-5 PubMed2463228

1988

Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread.

Applications

Unspecified application

Species

Unspecified reactive species

J Kovarík,A Rejthar,L Lauerová,B Vojtĕsek,J Bártková

View all publications

style

left-column

style

left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

style

left-column

style

right-column

Anti-Cytokeratin 18 抗体 [DA-7]

Anti-Cytokeratin 18 antibody [DA-7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DA-7] (AB669)

Flow Cytometry - Anti-Cytokeratin 18 antibody [DA-7] (AB669)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

下記製品にもご興味をお持ちいただけるかもしれません:

Human Cytokeratin 18 ELISA Kit

こちらの製品も推奨する理由

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

Abcam product promise

関連製品

Alternative Product

Primary Antibodies

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [EPR1626]

Alternative Product

Primary Antibodies

Anti-Cytokeratin 18 antibody [EPR1611]

Alternative Product

Primary Antibodies

Anti-Cytokeratin 18 antibody [EPR17347]

Alternative Product

Primary Antibodies

Anti-Cytokeratin 18 antibody [EPR1626]

Alternative Product

Primary Antibodies

Anti-Cytokeratin 18 antibody [E431-1]

Alternative Product

Primary Antibodies

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1]