Anti-Cytokeratin 18 抗体 [C-04] (ab668)
![Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)](https://www.abcam.co.jp/ps/products/0/ab668/Images/ab668-455403-anti-cytokeratin-18-antibody-flow-cytometry-cervix-human.jpg "Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)")
Key features and details
- Mouse monoclonal [C-04] to Cytokeratin 18
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Human, Mammals
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytokeratin 18 antibody [C-04]
Cytokeratin 18 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [C-04] to Cytokeratin 18 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-Pmore details -
種交差性
交差種: Human, Mammals -
免疫原
Tissue, cells or virus corresponding to Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431
Database link: P05783 -
ポジティブ･コントロール
- IHC-P: Human skin tissue. Flow Cyt (Intra): HCT 116 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
C-04 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab668の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 1 - 4 µg/ml.
Also see PMID 18946470. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-P | (14) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 1 - 4 µg/ml. Also see PMID 18946470. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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別名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)
Flow cytometric pattern (intracellular staining) of HeLa cells (Human epithelial cell line from cervix adenocarcinoma cell suspension) stained with ab668 (0.6 µg/ml concentration)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)](https://www.abcam.co.jp/ps/products/0/ab668/Images/ab668-455402-anti-cytokeratin-18-antibody-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [C-04] (ab668)Immunohistochemistry (paraffin-embedded sections) analysis of human kidney tissue labelling cytokeratin-18 with ab668 at 10µg/ml concentration.
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![Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)](https://www.abcam.co.jp/ps/products/0/ab668/Images/ab668-455399-anti-cytokeratin-18-antibody-flow-cytometry-hela-cells-human.jpg)
Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)Intracellular flow cytometric analysis (intracellular staining) of HeLa cells (human epithelial cell line from cervix adenocarcinoma cell suspension)stained with 0.6µg/ml ab668 (in red), and unstained HeLa cells (black).
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![Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)](https://www.abcam.co.jp/ps/products/0/ab668/Images/ab668-3603-anti-cytokeratin-18-antibody-c-04-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [C-04] (ab668)Overlay histogram showing HCT 116 (Human colorectal carcinoma cell line) cells stained with ab668 (red line).
The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab668, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT 116 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (166)
ab668 は 166 報の論文で使用されています。
- Rawat H et al. Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture models. Front Cell Dev Biol 11:1111684 (2023). PubMed: 37261075
- Wu JL et al. SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron. iScience 26:105995 (2023). PubMed: 36687314
- Toma C et al. In-Vitro Decidualization With Different Progesterone Concentration: Development of a Hormone-Responsive 3D Endometrial Tissue Using Rat Endometrial Tissues. Cureus 15:e49613 (2023). PubMed: 38033443
- Song M et al. FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation. J Reprod Dev 69:298-307 (2023). PubMed: 37779094
- Llorente A et al. MAF amplification licenses ERα through epigenetic remodelling to drive breast cancer metastasis. Nat Cell Biol 25:1833-1847 (2023). PubMed: 37945904