Anti-Cytokeratin 18 抗体 (ab52948)
Key features and details
- Rabbit polyclonal to Cytokeratin 18
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cytokeratin 18 antibody
Cytokeratin 18 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytokeratin 18 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide within Human Cytokeratin 18 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
(Peptide available asab215576) -
ポジティブ･コントロール
- A431 cell lysate, human urothelial carcinoma cells and HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| IHC-P |
Use at an assay dependent concentration.
|
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| ICC/IF | (1) |
1/250 - 1/500.
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| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
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ICC/IF
1/250 - 1/500. |
ターゲット情報
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機能
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
組織特異性
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
関連疾患
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 3875 Human
- Entrez Gene: 16668 Mouse
- Omim: 148070 Human
- SwissProt: P05783 Human
- SwissProt: P05784 Mouse
- Unigene: 406013 Human
- Unigene: 22479 Mouse
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別名
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
画像
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Western blot - Anti-Cytokeratin 18 antibody (ab52948)Anti-Cytokeratin 18 antibody (ab52948) at 1/50000 dilution + A431 cell lysate at 10 µg
Secondary
goat anti-rabbit HR at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Western blot - Anti-Cytokeratin 18 antibody (ab52948) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody (ab52948)ab52948 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody (ab52948)ab52948 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab52948 は 25 報の論文で使用されています。
- Lin JM et al. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons. Elife 11:N/A (2022). PubMed: 36111787
- Zambuto SG et al. Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels. ACS Biomater Sci Eng 8:3819-3830 (2022). PubMed: 35994527
- Alzheimer M et al. A three-dimensional intestinal tissue model reveals factors and small regulatory RNAs important for colonization with Campylobacter jejuni. PLoS Pathog 16:e1008304 (2020). PubMed: 32069333
- Hosoki A et al. The effect of radiation on the ability of rat mammary cells to form mammospheres. Radiat Environ Biophys 59:711-721 (2020). PubMed: 32996008
- Hahn M et al. Mesoscopic 3D imaging of pancreatic cancer and Langerhans islets based on tissue autofluorescence. Sci Rep 10:18246 (2020). PubMed: 33106532