Anti-Cytokeratin 17 抗体 [EPR1624Y] (ab51056)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1624Y] to Cytokeratin 17
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytokeratin 17 antibody [EPR1624Y]
Cytokeratin 17 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1624Y] to Cytokeratin 17 -
由来種
Rabbit -
特異性
The immunogen used for this product shares 78.6% homology with KRT36 and KRT24. Cross-reactivity with these proteins has not been confirmed experimentally. -
アプリケーション
適用あり: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Cytokeratin 17 aa 400-500 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HeLa cell lysate. ICC/IF: A431 cells. IP: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 2.47 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1624Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51056の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/250.
For unpurified use at 1/50 - 1/100 |
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Flow Cyt (Intra) |
1/20.
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WB |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
For unpurified use at 1/10000. |
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IP |
1/20.
For unpurified use at 1/30 |
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IHC-P |
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100. See IHC antigen retrieval protocols. |
特記事項 |
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ICC/IF
1/250. For unpurified use at 1/50 - 1/100 |
Flow Cyt (Intra)
1/20. |
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa). For unpurified use at 1/10000. |
IP
1/20. For unpurified use at 1/30 |
IHC-P
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/50 - 1/100. See IHC antigen retrieval protocols. |
ターゲット情報
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機能
May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. -
組織特異性
Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level). -
関連疾患
Defects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris. -
配列類似性
Belongs to the intermediate filament family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3872 Human
- Omim: 148069 Human
- SwissProt: Q04695 Human
- Unigene: 2785 Human
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別名
- 39.1 antibody
- CK 17 antibody
- CK-17 antibody
see all
画像
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Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056) at 1/1000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Exposure time: 1 secondBlocking and diluting buffer: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 17 with Purified ab51056 at 1:250 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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ab51056 (purified) at 1:20 dilution (1µg) immunoprecipitating Cytokeratin 17 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab51056 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51056 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with Purified ab51056 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical cancer tissue sections labeling Cytokeratin 17 with Purified ab51056 at 1:400 dilution (0.22 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab51056 は 14 報の論文で使用されています。
- Morin S et al. Eicosapentaenoic Acid Influences the Lipid Profile of an In Vitro Psoriatic Skin Model Produced with T Cells. Biomolecules 13:N/A (2023). PubMed: 37759812
- Xu J et al. A multifunctional composite hydrogel as an intrinsic and extrinsic coregulator for enhanced therapeutic efficacy for psoriasis. J Nanobiotechnology 20:155 (2022). PubMed: 35331238
- Heinzelmann K et al. Single-cell RNA sequencing identifies G-protein coupled receptor 87 as a basal cell marker expressed in distal honeycomb cysts in idiopathic pulmonary fibrosis. Eur Respir J 59:N/A (2022). PubMed: 35604813
- Castillo P et al. Novel insights linking BRCA1-IRIS role in mammary gland development to formation of aggressive PABCs: the case for longer breastfeeding. Am J Cancer Res 12:396-426 (2022). PubMed: 35141026
- Kantaputra P et al. Loss of Function TGFBR2 Variant as a Contributing Factor in Generalized Pustular Psoriasis and Adult-Onset Immunodeficiency. Genes (Basel) 14:N/A (2022). PubMed: 36672844