Anti-Cytokeratin 17 抗体 - Cytoskeleton Marker (ab53707)
Key features and details
- Rabbit polyclonal to Cytokeratin 17 - Cytoskeleton Marker
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytokeratin 17 antibody - Cytoskeleton Marker
Cytokeratin 17 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytokeratin 17 - Cytoskeleton Marker -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide derived from human Cytokeratin 17.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab53707 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53707の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| WB |
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| IHC-P |
1/50 - 1/100.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair. -
組織特異性
Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level). -
関連疾患
Defects in KRT17 are a cause of pachyonychia congenita type 2 (PC2) [MIM:167210]; also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth.
Defects in KRT17 are a cause of steatocystoma multiplex (SM) [MIM:184500]. SM is a disease characterized by round or oval cystic tumors widely distributed on the back, anterior trunk, arms, scrotum, and thighs.
Note=KRT16 and KRT17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris. -
配列類似性
Belongs to the intermediate filament family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3872 Human
- Omim: 148069 Human
- SwissProt: Q04695 Human
- Unigene: 2785 Human
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別名
- 39.1 antibody
- CK 17 antibody
- CK-17 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)ab53707 at 1/50 dilution staining Cytokeratin 17 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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Western blot - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)All lanes : Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707) at 1/500 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunising peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody - Cytoskeleton Marker (ab53707)ICC/IF image of ab53707 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53707, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (47)
ab53707 は 47 報の論文で使用されています。
- Wang G et al. Commensal microbiome promotes hair follicle regeneration by inducing keratinocyte HIF-1α signaling and glutamine metabolism. Sci Adv 9:eabo7555 (2023). PubMed: 36598999
- Higuchi S et al. BCL7B, a SWI/SNF complex subunit, orchestrates cancer immunity and stemness. BMC Cancer 23:811 (2023). PubMed: 37648998
- Sayed S et al. Isoxazole 9 (ISX9), a small molecule targeting Axin, activates Wnt/β-catenin signalling and promotes hair regrowth. Br J Pharmacol 180:1748-1765 (2023). PubMed: 36721985
- Maldonado H et al. Systemically administered wound-homing peptide accelerates wound healing by modulating syndecan-4 function. Nat Commun 14:8069 (2023). PubMed: 38057316
- Wu L et al. Interference KRT17 reverses doxorubicin resistance in triple-negative breast cancer cells by Wnt/β-catenin signaling pathway. Genes Genomics 45:1329-1338 (2023). PubMed: 37634232