AB233910

Anti-Cytokeratin 14 抗体 [SPM263] - C-terminal

Name: Anti-Cytokeratin 14 抗体 [SPM263] - C-terminal (ab233910) | Abcam
Brand: Abcam Limited
SKU: AB233910
Price: 74000 JPY
Availability: InStock

Anti-Cytokeratin 14 antibody [SPM263] - C-terminal

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(1 Publication)

Mouse Monoclonal Cytokeratin 14 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Keratin, type I cytoskeletal 14.

別名を表示する

Cytokeratin-14, Keratin-14, CK-14, K14, KRT14

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [SPM263] - C-terminal (AB233910)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [SPM263] - C-terminal (AB233910)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 14 using ab233910 at 1 μg/ml in immunohistochemical analysis.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

SPM263

アイソタイプ

IgG3

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human Keratin, type I cytoskeletal 14. The exact immunogen used to generate this antibody is proprietary information.

P02533

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

精製に関する特記事項

ab233910 purified from bioreactor concentrate by Protein A/G.

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.

Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

See full target information Keratin, type I cytoskeletal 14

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed32853177

2020

Contribution of plasma cells and B cells to hidradenitis suppurativa pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Johann E Gudjonsson,Lam C Tsoi,Feiyang Ma,Allison C Billi,K R van Straalen,A R J V Vossen,H H van der Zee,Paul W Harms,Rachael Wasikowski,Christine M Yee,Syed M Rizvi,Xianying Xing,Enze Xing,Olesya Plazyo,Chang Zeng,Matthew T Patrick,Margaret M Lowe,Richard E Burney,Jeffrey H Kozlow,Jill R Cherry-Bukowiec,Yanyun Jiang,Joseph Kirma,Stephan Weidinger,Kelly C Cushing,Michael D Rosenblum,Celine Berthier,Amanda S MacLeod,John J Voorhees,Fei Wen,J Michelle Kahlenberg,Emanual Maverakis,Robert L Modlin,Errol P Prens

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 14 抗体 [SPM263] - C-terminal

Anti-Cytokeratin 14 antibody [SPM263] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [SPM263] - C-terminal (AB233910)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

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組合せ製品

Assay Kits

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Abcam product promise