Anti-Cytokeratin 13 抗体 (ab79279)
Key features and details
- Goat polyclonal to Cytokeratin 13
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cytokeratin 13 antibody
Cytokeratin 13 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Cytokeratin 13 -
由来種
Goat -
特異性
ab79279 is expected to recognize the reported isoform a (NP_705694.2) only. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
C-TSNASGRRTSDVRRP
, corresponding to C terminal amino acids 444-458 of Human Cytokeratin 13 (NP_705694.2) -
ポジティブ･コントロール
- Human Lung, Skeletal Muscle and Skin lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab79279 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79279の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
| 特記事項 |
|---|
追加情報
Peptide ELISA: Antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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組織特異性
Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. -
関連疾患
Defects in KRT13 are a cause of white sponge nevus of cannon (WSN) [MIM:193900]. WSN is a rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
O-glycosylated; glycans consist of single N-acetylglucosamine residues. - Information by UniProt
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参照データベース
- Entrez Gene: 3860 Human
- Omim: 148065 Human
- SwissProt: P13646 Human
- Unigene: 654550 Human
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別名
- 47 kDa cytokeratin antibody
- CK-13 antibody
- CK13 antibody
see all
画像
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Western blot - Anti-Cytokeratin 13 antibody (ab79279)Anti-Cytokeratin 13 antibody (ab79279) at 0.1 µg/ml + Human Lung lysate (in RIPA buffer) at 35 µg
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr;149(Pt 4):961-71.; PMID: 12686638)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab79279 は 2 報の論文で使用されています。
- Tadokoro T et al. Dorso-ventral heterogeneity in tracheal basal stem cells. Biol Open 10:N/A (2021). PubMed: 34396394
- Montoro DT et al. A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature 560:319-324 (2018). PubMed: 30069044