Anti-Cytohesin 1 抗体 [EPR10050] (ab154847)
![Western blot - Anti-Cytohesin 1 antibody [EPR10050] (ab154847)](https://www.abcam.co.jp/ps/products/154/ab154847/Images/ab154847-161450-anti-cytohesin-1-antibody-epr10050-western-blot.jpg "Western blot - Anti-Cytohesin 1 antibody [EPR10050] (ab154847)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10050] to Cytohesin 1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cytohesin 1 antibody [EPR10050]
Cytohesin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10050] to Cytohesin 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Raji, Jurkat and HuT-78 cell lysates; Human colon tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10050 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154847の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 PH domain.
Contains 1 SEC7 domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9267 Human
- Omim: 182115 Human
- SwissProt: Q15438 Human
- Unigene: 191215 Human
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別名
- B2 1 antibody
- CLM1 antibody
- CTH 1 antibody
see all
画像
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Western blot - Anti-Cytohesin 1 antibody [EPR10050] (ab154847)All lanes : Anti-Cytohesin 1 antibody [EPR10050] (ab154847) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HuT-78 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 1 antibody [EPR10050] (ab154847)](https://www.abcam.co.jp/ps/products/154/ab154847/Images/ab154847-161449-anti-cytohesin-1-antibody-epr10050-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytohesin 1 antibody [EPR10050] (ab154847)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling Cytohesin 1 using ab154847 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Cytohesin 1 antibody [EPR10050] (ab154847)](https://www.abcam.co.jp/ps/products/154/ab154847/Images/ab154847-3-benefits-of-recombinant-antibodies.png)
Anti-Cytohesin 1 antibody [EPR10050] (ab154847)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154847 は 1 報の論文で使用されています。
- Chen X et al. Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Front Oncol 9:523 (2019). PubMed: 31293967