Anti-Cytochrome P450 4A/CYP4A11 抗体 (ab3573)
Key features and details
- Rabbit polyclonal to Cytochrome P450 4A/CYP4A11
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cytochrome P450 4A/CYP4A11 antibody
Cytochrome P450 4A/CYP4A11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytochrome P450 4A/CYP4A11 -
由来種
Rabbit -
特異性
The immunogen is homologous to rat cytochrome P450 4A2, 4A10, 4A12 and 4A14. Gene synonyms are Cyp4a11, Cyp4a1, Cyp4a8, and Cyp4a3, respectively. The specificity to these specific forms has not been confirmed experimentally. Please note nomenclature for specific forms may differ from mouse to rat. -
アプリケーション
適用あり: IHC-Fr, ICC/IF, IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Hamster, Cat, Dog, Human, Pig -
免疫原
Synthetic peptide corresponding to Rat Cytochrome P450 4A/CYP4A11 aa 400-500. The immunogen is homologous to rat cytochrome P450 4A2, A10, A12 and A14.
Database link: P08516 -
ポジティブ・コントロール
- WB: Rat liver tissue lysate, H-4-II-E and HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Whole antiserum -
一次抗体 備考
The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, & 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic & brain microvessels. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3573の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
1/20 - 1/200.
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IHC-P |
1/100 - 1/500.
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WB |
1/200 - 1/2000. Predicted molecular weight: 59 kDa.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/20 - 1/200. |
IHC-P
1/100 - 1/500. |
WB
1/200 - 1/2000. Predicted molecular weight: 59 kDa. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate, myristate and palmitate. Has little activity toward prostaglandins A1 and E1. Oxidizes arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE). -
組織特異性
Kidney and liver. -
配列類似性
Belongs to the cytochrome P450 family. -
細胞内局在
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1579 Human
- Entrez Gene: 24306 Rat
- Entrez Gene: 298423 Rat
- Entrez Gene: 50549 Rat
- Omim: 601310 Human
- SwissProt: Q02928 Human
- SwissProt: P14581 Rabbit
- SwissProt: P08516 Rat
see all -
別名
- 20-HETE synthase antibody
- 20-hydroxyeicosatetraenoic acid synthase antibody
- CP4AB_HUMAN antibody
see all
画像
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All lanes : Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573) at 1/1000 dilution
Lane 1 : Rat liver tissue lysate
Lane 2 : H-4-II-E cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 59 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?Chemiluminescent detection
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ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of H-4-II-E (rat) cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of HeLa cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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ab3573 labelling Cytochrome P450 4A/CYP4A11 (green) in the cytoplasm and membrane of PC12 cells (right), compared to control (left), by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody (ab3573)
ab3573 labelling Cytochrome P450 4A/CYP4A11 in the cytoplasm and membrane of Human liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab3573 は 16 報の論文で使用されています。
- Zhou F et al. The PPARα/CYP4A14 bile acid pathway is associated with lipid metabolism disorders caused by low birth weight with high-fat diet. Food Nutr Res 67:N/A (2023). PubMed: 36794015
- Deguise MO et al. SMN Depleted Mice Offer a Robust and Rapid Onset Model of Nonalcoholic Fatty Liver Disease. Cell Mol Gastroenterol Hepatol 12:354-377.e3 (2021). PubMed: 33545428
- Cui W et al. 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1a pathway: A translational study. Cell Prolif 54:e12964 (2021). PubMed: 33314534
- Han X et al. 20-HETE synthesis inhibition promotes cerebral protection after intracerebral hemorrhage without inhibiting angiogenesis. J Cereb Blood Flow Metab 39:1531-1543 (2019). PubMed: 29485354
- Lai YJ et al. Estrogen receptor a promotes Cav1.2 ubiquitination and degradation in neuronal cells and in APP/PS1 mice. Aging Cell N/A:e12961 (2019). PubMed: 31012223