AB248918

Anti-Cytochrome P450 4A/CYP4A11 抗体 [EPR8276] - BSA and Azide free

Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Cytochrome P450 4A/CYP4A11 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

CYP4A2, CYP4A11, Cytochrome P450 4A11, 20-hydroxyeicosatetraenoic acid synthase, CYP4AII, CYPIVA11, Cytochrome P-450HK-omega, Cytochrome P450HL-omega, Fatty acid omega-hydroxylase, Lauric acid omega-hydroxylase, Long-chain fatty acid omega-monooxygenase, 20-HETE synthase

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

This data was developed using ab140635, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

This data was developed using ab140635, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

This data was developed using ab140635, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab140635 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

This data was developed using ab140635, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Breast tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

This data was developed using ab140635, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

This data was developed using ab140635, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cytochrome P450 4A/CYP4A11 with ab140635 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

This data was developed using ab140635, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140635 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

This data was developed using ab140635, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] (<a href='/products/primary-antibodies/cytochrome-p450-4a-cyp4a11-antibody-epr8276-ab140635'>ab140635</a>) at 1/1000 dilution

Lane 1:

Mouse liver lysate at 10 µg

Lane 2:

Rat liver lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8276

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The immunogen is homologous to human cytochrome P450 4A11 and 4A22. The specificity to these specific forms has not been confirmed experimentally.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

製品の詳細

ab248918 is the carrier-free version of ab140635.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 4A also known as CYP4A11 plays an important role in the metabolism of fatty acids and arachidonic acid. This enzyme is a member of the cytochrome P450 superfamily and possesses a molecular mass of approximately 58 kDa. Expression of CYP4A11 occurs mainly in the liver and kidneys. It functions by introducing oxygen into various substrates facilitating their breakdown and further processing. CYP4A11 also exists as a monomer and does not require assembly into larger complexes to function efficiently.

Biological function summary

Cytochrome P450 enzymes like CYP4A11 contribute significantly to the metabolism of long-chain fatty acids. These metabolic processes generate substances important for cell signaling such as hydroxyeicosatetraenoic acids (HETEs). The enzyme also participates indirectly in blood pressure regulation through these metabolic products. Although CYP4A11 does not form part of a protein complex the metabolic byproducts have a role in several cellular mechanisms.

Pathways

CYP4A11 is integral to the fatty acid signaling pathway. Its action leads to the production of 20-HETE a metabolite involved in regulating vascular tone and renal function. Related proteins in this pathway include other cytochrome P450 family members particularly those involved in arachidonic acid metabolism like CYP2E1. Although not directly a part CYP4A11's metabolic activities feed into the pathways that control blood flow and pressure closely linking it to cardiovascular mechanisms.

CYP4A11's activity connects to hypertension and atherosclerosis. Dysregulation of its metabolic processes can lead to imbalances in fatty acid derivatives contributing to these conditions. Studies indicated a link between CYP4A11 and cardiovascular diseases with alterations in enzyme activity affecting blood pressure. The protein links to other P450 enzymes that similarly metabolize fatty acids and participate in maintaining cardiovascular health. Notably CYP4A11's influence on hypertension makes it a target for research into antihypertensive therapies.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins) (PubMed : 10553002, PubMed : 10660572, PubMed : 15611369, PubMed : 1739747, PubMed : 7679927, PubMed : 8914854). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed : 10553002, PubMed : 10660572, PubMed : 15611369, PubMed : 1739747, PubMed : 7679927, PubMed : 8914854). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids, the catalytic efficiency decreasing in the following order : dodecanoic > tetradecanoic > (9Z)-octadecenoic > (9Z,12Z)-octadecadienoic > hexadecanoic acid (PubMed : 10553002, PubMed : 10660572). Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver (PubMed : 15611369, PubMed : 1739747, PubMed : 7679927, PubMed : 8914854). Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed : 10620324, PubMed : 10660572, PubMed : 15611369). Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency (PubMed : 7679927). May contribute to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening (PubMed : 18182499). Omega-hydroxylates (9R,10S)-epoxy-octadecanoate stereoisomer (PubMed : 15145985). Plays a minor role in omega-oxidation of long-chain 3-hydroxy fatty acids (PubMed : 18065749). Has little activity toward prostaglandins A1 and E1 (PubMed : 7679927).

See full target information CYP4A11

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytochrome P450 4A/CYP4A11 抗体 [EPR8276] - BSA and Azide free

Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

Western blot - Anti-Cytochrome P450 4A/CYP4A11 antibody [EPR8276] - BSA and Azide free (AB248918)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise