Anti-Cytochrome P450 3A4/CYP3A4 抗体 (ab3572)
Key features and details
- Rabbit polyclonal to Cytochrome P450 3A4/CYP3A4
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cytochrome P450 3A4/CYP3A4 antibody
Cytochrome P450 3A4/CYP3A4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cytochrome P450 3A4/CYP3A4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein (His-tag) corresponding to Human Cytochrome P450 3A4/CYP3A4. Purified, His-tagged, full length human P450 3A4 fusion protein.
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ポジティブ･コントロール
- WB: Rat and mouse liver lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3572の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).
A 46kDa band is detected in rat liver extracts. |
| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa). A 46kDa band is detected in rat liver extracts. |
ターゲット情報
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機能
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. -
組織特異性
Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). -
配列類似性
Belongs to the cytochrome P450 family. -
翻訳後修飾
Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). -
細胞内局在
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1576 Human
- Omim: 124010 Human
- SwissProt: P08684 Human
- Unigene: 728751 Human
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別名
- 1,8-cineole 2-exo-monooxygenase antibody
- Albendazole monooxygenase antibody
- Albendazole sulfoxidase antibody
see all
画像
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution
Lane 1 : Mouse liver tissue extract
Lane 2 : Rat liver tissue extract
Lane 3 : Mouse stomach tissue extract
Lane 4 : Rat stomach tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.Electrophoresed on 4-12% BisTris gel transferred onto a nitrocellulose membrane
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)All lanes : Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572) at 1/2000 dilution
Lane 1 : Mouse liver with Skimmed milk
Lane 2 : Rat liver with Skimmed milk
Lysates/proteins at 30 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 57 kDa -
Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)Western blot of Cytochrome P450 3A4/CYP3A4 on rat liver extract using ab3572.
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Western blot - Anti-Cytochrome P450 3A4/CYP3A4 antibody (ab3572)ab3572 at a dilution of 1/2000 staining Cytochrome P450 3A4/CYP3A4 in rat liver extract by Western blot.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (31)
ab3572 は 31 報の論文で使用されています。
- Creisher PS et al. Adverse outcomes in SARS-CoV-2-infected pregnant mice are gestational age-dependent and resolve with antiviral treatment. J Clin Invest 133:N/A (2023). PubMed: 37581940
- Ma L et al. Role of circadian clock in the chronoefficacy and chronotoxicity of clopidogrel. Br J Pharmacol 180:2973-2988 (2023). PubMed: 37403641
- Yang J et al. Hepatoprotective Effects of Phloridzin against Isoniazid-Rifampicin Induced Liver Injury by Regulating CYP450 and Nrf2/HO-1 Pathway in Mice. Chem Pharm Bull (Tokyo) 70:805-811 (2022). PubMed: 36070932
- Bai X et al. Regulation of CYP450 and drug transporter mediated by gut microbiota under high-altitude hypoxia. Front Pharmacol 13:977370 (2022). PubMed: 36188572
- Wang Q et al. Mechanisms Underlying the Differences in the Pharmacokinetics of Six Active Constituents of Huangqi Liuyi Decoction between Normal and Diabetic Nephropathy Mouse Models. Evid Based Complement Alternat Med 2022:2481654 (2022). PubMed: 36285162