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リコンビナントRabMAb

Anti-Cytochrome p450 2C19 抗体 [EPR6577] - C-terminal (ab185213)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
  • Immunoprecipitation - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
  • Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
  • Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
  • Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6577] to Cytochrome p450 2C19 - C-terminal
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal
    Cytochrome p450 2C19 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR6577] to Cytochrome p450 2C19 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IP, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa cells, 293 cells, Human fetal liver tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185213の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
IP
1/40 - 1/60.
ICC/IF
1/250.
特記事項
Flow Cyt (Intra)
1/240.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
IP
1/40 - 1/60.
ICC/IF
1/250.

ターゲット情報

  • 機能

    Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine.

  • 配列類似性

    Belongs to the cytochrome P450 family.

  • 細胞内局在

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • (R)-limonene 6-monooxygenase antibody
      • (S)-limonene 6-monooxygenase antibody
      • (S)-limonene 7-monooxygenase antibody
      • CP2CJ_HUMAN antibody
      • CPCJ antibody
      • CYP2C antibody
      • CYP2C19 antibody
      • CYPIIC17 antibody
      • CYPIIC19 antibody
      • Cytochrome P-450 II C antibody
      • Cytochrome P450 2C19 antibody
      • Cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 19 antibody
      • Cytochrome P450-11A antibody
      • Cytochrome P450-254C antibody
      • Flavoprotein-linked monooxygenase antibody
      • Mephenytoin 4 hydroxylase antibody
      • Mephenytoin 4-hydroxylase antibody
      • Microsomal monooxygenase antibody
      • P450-11A antibody
      • P45011A antibody
      • P450C2C antibody
      • S-mephenytoin 4-hydroxylase antibody
      • Xenobiotic monooxygenase antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
      Flow Cytometry (Intracellular) - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

      Intracellular flow cytometric analysis of2% paraformaldehyde fixed HeLa cells labeling Cytochrome P450 2C19 with ab185213 at 1/240 (red) or Rabbit monoclonal IgG (green)followed byGoat anti rabbit IgG (FITC) at 1/150. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

      Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling Cytochrome P450 2C19 with ab185213 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 and counterstained with Dapi.

    • Immunoprecipitation - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
      Immunoprecipitation - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

      Immunoprecipitation analysis of 293 cell lysate immunoprecipitated for Cytochrome P450 2C19 using ab185213 at 1/50 dilution (Lane 1). A aAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution was used as secondary. Lane 2: PBS instead of 293 lysates

    • Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
      Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
      All lanes : Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

      Predicted band size: 56 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
      Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
      Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 56 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?

    • Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
      Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

    参考文献 (1)

    ab185213 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185213 は 1 報の論文で使用されています。

    • Wang P  et al. The Long Noncoding RNA Hepatocyte Nuclear Factor 4α Antisense RNA 1 Negatively Regulates Cytochrome P450 Enzymes in Huh7 Cells via Histone Modifications. Drug Metab Dispos 49:361-368 (2021). PubMed: 33674270

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