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リコンビナントRabMAb

Anti-Cytochrome P450 1A2 抗体 [EPR6138(2)] (ab151728)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (5)

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Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
  • Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
  • Flow Cytometry (Intracellular) - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
  • Flow Cytometry (Intracellular) - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
  • Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6138(2)] to Cytochrome P450 1A2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

こちらの製品もご検討ください

Western blot
Product image
Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (ab116027)
一次抗体
Product image
Anti-Villin antibody [EPR3491(3)] (ab133510)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

関連製品

製品の概要

  • 製品名

    Anti-Cytochrome P450 1A2 antibody [EPR6138(2)]
    Cytochrome P450 1A2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR6138(2)] to Cytochrome P450 1A2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
    適用なし: IHC-P or IP
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide within Human Cytochrome P450 1A2 aa 200-300. The exact sequence is proprietary.

  • ポジティブ・コントロール

    • WB: Caco2, HepG2, HeLa and A549 cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): MCF7 cells.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at -20ºC.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR6138(2)
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Neuroscience
    • Processes

関連製品

  • Alternative Versions

    • Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] - BSA and Azide free (ab249045)
    • Alexa Fluor® 568 Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab312665)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151728の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
ICC/IF
1/200 - 1/500.
特記事項
Flow Cyt (Intra)
1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
ICC/IF
1/200 - 1/500.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.
  • 組織特異性

    Liver.
  • 配列類似性

    Belongs to the cytochrome P450 family.
  • 細胞内局在

    Endoplasmic reticulum membrane. Microsome membrane.
  • Target information above from: UniProt accession P05177 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 1544 Human
    • Omim: 124060 Human
    • SwissProt: P05177 Human
    • Unigene: 1361 Human
    • 別名

      • Aryl hydrocarbon hydroxylase antibody
      • CP 12 antibody
      • CP12 antibody
      • CP1A2_HUMAN antibody
      • CYP1A2 antibody
      • CYPIA2 antibody
      • Cytochrome P(3)450 antibody
      • Cytochrome P450 1A2 antibody
      • Cytochrome P450 4 antibody
      • Cytochrome P450 family 1 polypeptide 2 antibody
      • Cytochrome P450 family 1 subfamily A polypeptide 2 antibody
      • Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 antibody
      • Cytochrome P450-P3 antibody
      • Cytochrome P4501A2 antibody
      • Dioxin inducable P3 450 antibody
      • Flavoprotein linked monooxygenase antibody
      • Flavoprotein-linked monooxygenase antibody
      • Microsomal monooxygenase antibody
      • P(3)450 antibody
      • P3 450 antibody
      • P450 4 antibody
      • P450 form 4 antibody
      • P450 P3 antibody
      • P450(PA) antibody
      • Xenobiotic monooxygenase antibody
      see all

    画像

    • Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728) at 1/2000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytochrome P450 1A2 with purified ab151728 at 1/200 dilution (9.4 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Western blot - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      All lanes : Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728) at 1/1000 dilution ((unpurified))

      Lane 1 : Caco2 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa

    • Flow Cytometry (Intracellular) - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Flow Cytometry (Intracellular) - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

      Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Cytochrome P450 1A2 with purified ab151728 at 1/200 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

      Immunofluorescent analysis of HeLa cells labeling Cytochrome P450 1A2 with unpurified ab151728 at 1/250 dilution.

    • Flow Cytometry (Intracellular) - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Flow Cytometry (Intracellular) - Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

      Overlay histogram showing MCF7 cells stained with unpurified ab151728 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151728, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

       

       

    • Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)
      Anti-Cytochrome P450 1A2 antibody [EPR6138(2)] (ab151728)

    プロトコール

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (5)

    ab151728 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab151728 は 5 報の論文で使用されています。

    • Wang P  et al. The Long Noncoding RNA Hepatocyte Nuclear Factor 4α Antisense RNA 1 Negatively Regulates Cytochrome P450 Enzymes in Huh7 Cells via Histone Modifications. Drug Metab Dispos 49:361-368 (2021). PubMed: 33674270
    • Zhou Y  et al. lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis. Open Life Sci 16:969-980 (2021). PubMed: 34595348
    • Collins DP  et al. In vitro Differentiation of Human TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells. Hepat Med 12:79-92 (2020). PubMed: 32607015
    • Aravalli RN  et al. Hepatic Differentiation of Marmoset Embryonic Stem Cells and Functional Characterization of ESC-Derived Hepatocyte-Like Cells. Hepat Med 12:15-27 (2020). PubMed: 32104112
    • Gill P  et al. MicroRNA regulation of CYP 1A2, CYP3A4 and CYP2E1 expression in acetaminophen toxicity. Sci Rep 7:12331 (2017). WB . PubMed: 28951593

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Inquiry: Did this Ab cross react with others CYP1A notably CYP1A1 ?

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2013

    Answer

    Unfortunately, I am sorry to confirm we did not specifically test ab151728 CYP1A antibody for this cross reactivity. However, we did a BLAST with the immunogen sequence and this indicates that theoretically this antibody will not cross react with other CYP1A proteins. We ran an alignment, specifically with the sequence for CYP1A1, and the homology is very low (not significant).

    Read More

    Abcam Scientific Support

    Answered on May 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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